The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form

A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles / Hamidi-Asl, Ezat; Raoof, Jahan Bakhsh; Hejazi, Mohammad Saeid; Sharifi, Simin; Golabi, Seyed Mahdi; Palchetti, Ilaria; Mascini, Marco. - In: ELECTROANALYSIS. - ISSN 1040-0397. - STAMPA. - 27:(2015), pp. 1378-1386. [10.1002/elan.201400660]

A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles

PALCHETTI, ILARIA;
2015

Abstract

The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form
2015
27
1378
1386
Hamidi-Asl, Ezat; Raoof, Jahan Bakhsh; Hejazi, Mohammad Saeid; Sharifi, Simin; Golabi, Seyed Mahdi; Palchetti, Ilaria; Mascini, Marco
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1089345
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