Infusions and decoctions of three chemotypes of Lippia alba (Mill.) N. E. Brown (Verbenaceae) were investigated for their quantitative profiles by HPLC–DAD–ESI-MS analyses. An RP-HPLC method was developed which permitted the quality control of the preparations. The correct choice of the column allowed the detailed characterization of the constituents in a total analysis time of 35 min. The HPLC method was accordingly validated for linearity range, LOD, LOQ, accuracy and precision. For the quantitative analysis the three major phytochemical groups were taken into consideration, namely iridoids, phenylpropanoids and flavonoids. Comparative quantitative analyses revealed significant differences among the chemotypes that should be taken into account in the uses of the herbal teas. The developed HPLC-UV assay proved to be an efficient and alternative method for the discrimination of the three chemotypes. This is the first report of detailed analysis of the chemical composition of the constituents of L. alba chemotypes’ teas.

A validated HPLC method for the analysis of herbal teas from three Lippia alba chemotypes of Brazilian / Patrícia Timóteo; Anastasia Karioti ; Suzana G. Leitão; Franco Francesco Vincieri; Anna Rita Bilia. - In: ELECTRONIC JOURNAL OF ENVIRONMENTAL, AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 1579-4377. - ELETTRONICO. - (2015), pp. 366-373.

A validated HPLC method for the analysis of herbal teas from three Lippia alba chemotypes of Brazilian

BILIA, ANNA RITA
2015

Abstract

Infusions and decoctions of three chemotypes of Lippia alba (Mill.) N. E. Brown (Verbenaceae) were investigated for their quantitative profiles by HPLC–DAD–ESI-MS analyses. An RP-HPLC method was developed which permitted the quality control of the preparations. The correct choice of the column allowed the detailed characterization of the constituents in a total analysis time of 35 min. The HPLC method was accordingly validated for linearity range, LOD, LOQ, accuracy and precision. For the quantitative analysis the three major phytochemical groups were taken into consideration, namely iridoids, phenylpropanoids and flavonoids. Comparative quantitative analyses revealed significant differences among the chemotypes that should be taken into account in the uses of the herbal teas. The developed HPLC-UV assay proved to be an efficient and alternative method for the discrimination of the three chemotypes. This is the first report of detailed analysis of the chemical composition of the constituents of L. alba chemotypes’ teas.
2015
366
373
Patrícia Timóteo; Anastasia Karioti ; Suzana G. Leitão; Franco Francesco Vincieri; Anna Rita Bilia
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1090255
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