The accurate determination of carboxylic acids in biological matrices is crucial for understanding metabolic pathways related to plant diseases, as well as plant growth and development. Here, the determination of several organic acids was achieved by GC/MS, following a single-step aqueous derivatization with triethyloxonium tetrafluoroborate. For the utmost precision, quantitation was performed based on isotope dilution formalism by using deuterated internal standards. Linearity, repeatability, recovery, limit of detection and quantitation were evaluated for each analyte under investigation, along with the plant matrix effects.
Determination of carboxylic acids in biological matrices by GC/MS following single-step aqueous derivatization with triethyloxonium tetrafluoroborate / Ferraro, D.; Onor, M.; Campanella, B.; Tegli, S.; Bramanti, E.; Giannarelli, S.; D’Ulivo, A.; Pagliano, E.. - ELETTRONICO. - (2015), pp. 277-277. (Intervento presentato al convegno Colloquium Spectroscopicum Internationale XXXIX tenutosi a Figueira da Foz, Coimbra, Portugal nel 30 AUGUST - 3 SEPTEMBER 2015).
Determination of carboxylic acids in biological matrices by GC/MS following single-step aqueous derivatization with triethyloxonium tetrafluoroborate
TEGLI, STEFANIA;
2015
Abstract
The accurate determination of carboxylic acids in biological matrices is crucial for understanding metabolic pathways related to plant diseases, as well as plant growth and development. Here, the determination of several organic acids was achieved by GC/MS, following a single-step aqueous derivatization with triethyloxonium tetrafluoroborate. For the utmost precision, quantitation was performed based on isotope dilution formalism by using deuterated internal standards. Linearity, repeatability, recovery, limit of detection and quantitation were evaluated for each analyte under investigation, along with the plant matrix effects.File | Dimensione | Formato | |
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