The efficiency of satellite cells in repairing adult skeletal muscle is limited in case of severe damage [1]. To improve the muscle repair potential, cell therapy strategies have been proposed with the use of bone marrow mesenchymal stromal cells (BM-MSCs) [2], even if their clinical application is hampered by criticisms, including scarce survival in the host tissue and the need to avoid animal serum contamination during expansion. In this context, platelet rich plasma (PRP) may offer several advantages representing an optimal substitute of animal serum in vitro and potentially providing beneficial therapeutic effects in vivo in the tissue injury site. Controversial data are present regarding its application in skeletal muscle injuries [3]. Here we evaluated in an in vitro model: i) the effect of PRP on C2C12 myoblasts, murine satellite cells and BM-MSCs in terms of viability, survival, proliferation and myogenic differentiation and ii) the effect of PRP in combination with BM-MSCs in sustaining and promoting myogenic differentiation. We found that PRP induced an increase of myoblast and BM-MSC survival, viability and proliferation, promoted C2C12 myoblast differentiation as evaluated by the analysis of MyoD, myogenin, α-sarcomeric actin, MMP-2 and MMP-9, as well as satellite cells activation. Notably PRP/BM-MSCs was more effective than PRP alone. Within the limitations of an in vitro study, our data suggest that t PRP/BM-MSCs combination can be considered as a valuable tool for skeletal muscle regenerative medicine. [1] Costamagna et al. (2015) Adult Stem Cells and Skeletal Muscle Regeneration.Curr Gene Ther,15:348 [2] Sassoli C et al.(2014) Mesenchymal stromal cell secreted sphingosine 1-phosphate (S1P) exerts a stimulatory effect on skeletal myoblast proliferation. PLoS One. 29:108662 [3] Andia I, Abate M. (2015) Platelet-rich plasma in the treatment of skeletal muscle injuries. Expert Opin Biol Ther;15:987
Combined use in vitro of bone marrow-derived mesenchymal stromal cells (BM-MSCs) and Platelet Rich Plasma (PRP) as possible therapeutic tool for skeletal muscle repair/regeneration / Vallone, Larissa; Sassoli, Chiara; Tani, Alessia; Chellini, Flaminia; Nosi, Daniele; Zecchi-Orlandini, Sandra. - ELETTRONICO. - (2017), pp. 0-0. (Intervento presentato al convegno ABCD congress (The Biennial Congress of the Italian Association of Cell Biology and Differentiation) 2017. Associazione di biologia cellulare e differenziamento. tenutosi a Bologna Savoia hotel Regency (Via del pilastro 2 40127) nel 21-23 September 2017.).
Combined use in vitro of bone marrow-derived mesenchymal stromal cells (BM-MSCs) and Platelet Rich Plasma (PRP) as possible therapeutic tool for skeletal muscle repair/regeneration
VALLONE, LARISSA;SASSOLI, CHIARA;TANI, ALESSIA;CHELLINI, FLAMINIA;NOSI, DANIELE;ZECCHI, SANDRA
2017
Abstract
The efficiency of satellite cells in repairing adult skeletal muscle is limited in case of severe damage [1]. To improve the muscle repair potential, cell therapy strategies have been proposed with the use of bone marrow mesenchymal stromal cells (BM-MSCs) [2], even if their clinical application is hampered by criticisms, including scarce survival in the host tissue and the need to avoid animal serum contamination during expansion. In this context, platelet rich plasma (PRP) may offer several advantages representing an optimal substitute of animal serum in vitro and potentially providing beneficial therapeutic effects in vivo in the tissue injury site. Controversial data are present regarding its application in skeletal muscle injuries [3]. Here we evaluated in an in vitro model: i) the effect of PRP on C2C12 myoblasts, murine satellite cells and BM-MSCs in terms of viability, survival, proliferation and myogenic differentiation and ii) the effect of PRP in combination with BM-MSCs in sustaining and promoting myogenic differentiation. We found that PRP induced an increase of myoblast and BM-MSC survival, viability and proliferation, promoted C2C12 myoblast differentiation as evaluated by the analysis of MyoD, myogenin, α-sarcomeric actin, MMP-2 and MMP-9, as well as satellite cells activation. Notably PRP/BM-MSCs was more effective than PRP alone. Within the limitations of an in vitro study, our data suggest that t PRP/BM-MSCs combination can be considered as a valuable tool for skeletal muscle regenerative medicine. [1] Costamagna et al. (2015) Adult Stem Cells and Skeletal Muscle Regeneration.Curr Gene Ther,15:348 [2] Sassoli C et al.(2014) Mesenchymal stromal cell secreted sphingosine 1-phosphate (S1P) exerts a stimulatory effect on skeletal myoblast proliferation. PLoS One. 29:108662 [3] Andia I, Abate M. (2015) Platelet-rich plasma in the treatment of skeletal muscle injuries. Expert Opin Biol Ther;15:987
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