In this study, the utility of the ITS region as a means of accurately identifying GTD fungi was assessed in an international inter-laboratory comparison comprising of researchers participating in the EU cost action on GTD (manaGTD). Twelve samples of fungal DNA associated with GTD fungi were distributed to researchers in 14 laboratories. The researchers were asked to use their own DNA barcoding protocols to identify the GTD species using phylogenetic markers for the ITS, histone and elongation factor regions. The results and the relative robustness of DNA barcoding approaches for GTD fungi are discussed.
|Titolo:||An inter-laboratory comparison of DNA barcoding protocols for identifying fungi involved in grapevine trunk disease|
|Anno di registrazione:||2017|
|Autori di Ateneo:|
|Autori:||Woodhall, J.; Perkins, K.; Reis, P.; Rego, C.; Aves, A.; Eichmeier, A.; Romanazzi, G.; Kovacs, C.; Karaffa, E.; Váczy, K. Z.; Mugnai, L.; Carlucci, A.; Muñoz, R.; Gramaje, D.; Armengol, J.; Pinto, C.; Fontaine, F.|
|Appare nelle tipologie:||1c - Abstract su rivista|