Cerebrospinal fluid amounts of HLA-G in dimeric form are strongly associated to patients with MRI inactive multiple sclerosis

Fainardi, Enrico; Bortolotti, Daria; Bolzani, Silvia; Castellazzi, Massimiliano; Tamborino, Carmine; Roversi, Gloria; Baldi, Eleonora; Caniatti, Maria Luisa; Casetta, Ilaria; Gentili, Valentina; Granieri, Enrico; Rizzo, Roberta; Tola, M. R.; Dallocchio, F.; Bellini, T.; Rotola, A.; Di Luca, D.; Seraceni, S.; Contini, C.; Sabbioni, S.; Negrini, M.; Tognon, M.; Antonelli, T.; Groppo, E.; Gentile, M.; Ceruti, S.; Manfrinato, M. R.; Trentini, A.; Miotto, E.; Ferracin, M.; Mazzoni, Elisa; Pietrobon, S.; Masini, I.; Rotondo, J. C.; Martini, F.; Baruzzi, A.; Roberto D'Alessandro, R.; Michelucci, R.; Salvi, F.; Stecchi, S.; Scandellari, C.; Terzano, G.; Granella, F.; Nichelli, P.; Sola, P.; Ferraro, D.; Vitetta, F.; Simone, A. M.; Bedin, R.; Marcello, N.; Motti, L.; Montepietra, S.; Guidetti, D.; Immovilli, P.; Montanari, E.; Pesci, I.; Guareschi, A.; Greco, G.; Santangelo, M.; Mauro, A. M.; Malagã¹, S.; Rasi, F.; Spadoni, M.; Galeotti, M.; Fiorani, L.; Neri, W.; Ravasio, A.; Pasquinelli, M.; Gutman, S.; Monaldini, C.

doi:10.1177/1352458515590647

BACKGROUND: The relevance of human leukocyte antigen (HLA)-G in dimeric form in multiple sclerosis (MS) is still unknown. OBJECTIVE: To investigate the contribution of cerebrospinal fluid (CSF) HLA-G dimers in MS pathogenesis. METHODS: CSF amounts of 78-kDa HLA-G dimers were measured by western blot analysis in 80 MS relapsing-remitting MS (RRMS) patients and in 81 inflammatory and 70 non-inflammatory controls. RESULTS: CSF amounts of 78 kDa HLA-G dimers were more frequent in RRMS than in inflammatory (p<0.01) and non-inflammatory controls (p<0.001) and in magnetic resonance imaging (MRI) inactive than in MRI active RRMS (p<0.00001). CONCLUSION: Our findings suggest that HLA-G dimers may be implicated in termination of inflammatory response occurring in MS.

Cerebrospinal fluid amounts of HLA-G in dimeric form are strongly associated to patients with MRI inactive multiple sclerosis / Fainardi, E., Bortolotti, D., Bolzani, S., Castellazzi, M., Tamborino, C., Roversi, G., Baldi, E., Caniatti, M.L., Casetta, I., Gentili, V., Granieri, E., Rizzo, R., Tola, M.R., Dallocchio, F., Bellini, T., Rotola, A., Di Luca, D., Seraceni, S., Contini, C., Sabbioni, S., et al.. - In: MULTIPLE SCLEROSIS. - ISSN 1352-4585. - ELETTRONICO. - 22:(2016), pp. 245-249. [10.1177/1352458515590647]