Here we present a study of single processive myosin motors based on the combination of optical tweezers and fluorescence imaging techniques. Ultrafast force-clamp spectroscopy [1] is applied to study the dependence of the chemo-mechanical properties of myosin on load and on ATP concentration. On the other hand, single molecule localization through FIONA (Fluorescence Imaging with One Nanometer Accuracy) [2, 3] is applied to in vitro motility assay to measure parameters such as the runlength, the velocity and the step size of single myosin motors, labelled with Quantum Dots.
Single molecule study of processive myosin motors / Gardini, L.; Pavone, F. S.; Capitanio, M.. - ELETTRONICO. - 2016:(2016), pp. 0-0. (Intervento presentato al convegno 18th Italian National Conference on Photonic Technologies, Fotonica 2016 tenutosi a ita nel 2016).
Single molecule study of processive myosin motors
Gardini, L.;Pavone, F. S.;Capitanio, M.
2016
Abstract
Here we present a study of single processive myosin motors based on the combination of optical tweezers and fluorescence imaging techniques. Ultrafast force-clamp spectroscopy [1] is applied to study the dependence of the chemo-mechanical properties of myosin on load and on ATP concentration. On the other hand, single molecule localization through FIONA (Fluorescence Imaging with One Nanometer Accuracy) [2, 3] is applied to in vitro motility assay to measure parameters such as the runlength, the velocity and the step size of single myosin motors, labelled with Quantum Dots.
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