Light-sheet microscopy enables whole mouse brain imaging in association with clearing methodologies. Here, we present a pipeline for optimal investigation of the vascular component, which offers improved image quality for morphological analysis.
Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy / Di Giovanna, Antonino Paolo; Tibo, Alessandro; Silvestri, Ludovico; Mã¼llenbroich, Marie Caroline; Costantini, Irene; Sacconi, Leonardo; Frasconi, Paolo; Pavone, Francesco Saverio. - STAMPA. - 2017:(2017), pp. 104140N-104140N-3. (Intervento presentato al convegno European Conference on Biomedical Optics, ECBO 2017 tenutosi a ICM-International Congress Center Munich, deu nel 2017) [10.1117/12.2285044].
Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy
DI GIOVANNA, ANTONINO PAOLO;TIBO, ALESSANDRO;Silvestri, Ludovico;Costantini, Irene;Sacconi, Leonardo;Frasconi, Paolo;Pavone, Francesco Saverio
2017
Abstract
Light-sheet microscopy enables whole mouse brain imaging in association with clearing methodologies. Here, we present a pipeline for optimal investigation of the vascular component, which offers improved image quality for morphological analysis.
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