A cohort of 19 patients affected by chronic venous ulcers was recruited from our centre. A 4-mm punch biopsy from wound bed was taken before application of ALA 20% gel and repeated one hour after the first PDT irradiation. We observed a significant and progressive reduction of wounds mean volumes right after three ALA-PDT sessions (once per week; 4479.9 +/- 345.5 mm3 vs 3459 +/- 190.3 mm3, p<0.01). On immunofluorescence staining from biopsy specimens, we observed a change in all tested stains of post treatment specimens compared to pre-treatment ones. An increase of plasmacytoid dendritic cells (from 69 +/- 22 cells/0.018 mm2 to 136 +/- 27 cells/0.018 mm2, p<0.0001); MHC-II expression (260.3 +/- 99.7 Red, Green, Blue [RGB 0-255] to 370.2 +/- 162.6 RGB (0-255), p<0.01), TNF-alpha positive mast cells expression (4 +/- 0.3 cells/0.018 mm2 to 6 +/- 0.4 cells/0.018 mm2, p<0.001), TGF-beta expression (59.8 +/- 23.2 RGB (0-255)/cell vs 137.3 +/- 56.6 RGB (0-255)/cell, p<0,01) and CD4+/CD25+ Treg cells (3 +/- 1 cells/0.018 mm2 vs 20 +/- 10 cells/0.018 mm2, p<0.001) was observed. An increase of TGF-beta was correlated in a statistical significant manner with a reduction of wounds’ mean volumes.

ALA-PDT exerts beneficial effects on chronic venous ulcers by inducing changes in inflammatory microenvironment, especially through increased TGF-beta release: a pilot clinical and translational study / Vieri, Grandi; Stefano, Bacci; Alessandro, Corsi; Maurizio, Sessa; Elisa, Puliti; Nicoletta, Murciano; Francesca, Scavone; Pietro, Cappugi; Nicola, Pimpinelli. - In: PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY. - ISSN 1572-1000. - STAMPA. - 21:(2018), pp. 252-256. [10.1016/j.pdpdt.2017.12.012]

ALA-PDT exerts beneficial effects on chronic venous ulcers by inducing changes in inflammatory microenvironment, especially through increased TGF-beta release: a pilot clinical and translational study

Vieri Grandi
Writing – Review & Editing
;
Stefano Bacci
Writing – Review & Editing
;
Alessandro Corsi
Membro del Collaboration Group
;
Elisa Puliti
Investigation
;
Nicoletta Murciano
Investigation
;
Francesca Scavone
Investigation
;
Pietro Cappugi
Resources
;
Nicola Pimpinelli
Resources
2018

Abstract

A cohort of 19 patients affected by chronic venous ulcers was recruited from our centre. A 4-mm punch biopsy from wound bed was taken before application of ALA 20% gel and repeated one hour after the first PDT irradiation. We observed a significant and progressive reduction of wounds mean volumes right after three ALA-PDT sessions (once per week; 4479.9 +/- 345.5 mm3 vs 3459 +/- 190.3 mm3, p<0.01). On immunofluorescence staining from biopsy specimens, we observed a change in all tested stains of post treatment specimens compared to pre-treatment ones. An increase of plasmacytoid dendritic cells (from 69 +/- 22 cells/0.018 mm2 to 136 +/- 27 cells/0.018 mm2, p<0.0001); MHC-II expression (260.3 +/- 99.7 Red, Green, Blue [RGB 0-255] to 370.2 +/- 162.6 RGB (0-255), p<0.01), TNF-alpha positive mast cells expression (4 +/- 0.3 cells/0.018 mm2 to 6 +/- 0.4 cells/0.018 mm2, p<0.001), TGF-beta expression (59.8 +/- 23.2 RGB (0-255)/cell vs 137.3 +/- 56.6 RGB (0-255)/cell, p<0,01) and CD4+/CD25+ Treg cells (3 +/- 1 cells/0.018 mm2 vs 20 +/- 10 cells/0.018 mm2, p<0.001) was observed. An increase of TGF-beta was correlated in a statistical significant manner with a reduction of wounds’ mean volumes.
2018
21
252
256
Goal 3: Good health and well-being for people
Vieri, Grandi; Stefano, Bacci; Alessandro, Corsi; Maurizio, Sessa; Elisa, Puliti; Nicoletta, Murciano; Francesca, Scavone; Pietro, Cappugi; Nicola, Pi...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1106481
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