To prepare the ESA (European Space Agency) spaceflight project “Wound healing and Sutures in Unloading Conditions”, we studied mechanisms of apoptosis in wound healing models based on ex vivo skin tissue cultures, kept for 10 days alive in serum-free DMEM/F12 medium supplemented with bovine serum albumin, hydrocortisone, insulin, ascorbic acid and antibiotics at 32 ◦C. The overall goal is to test: (i) the viability of tissue specimens; (ii) the gene expression of activators and inhibitors of apoptosis and extracellular matrix components in wound and suture models; and (iii) to design analytical protocols for future tissue specimens after post-spaceflight download.
Preparation of a spaceflight: Apoptosis search in sutured wound healing models / Riwaldt, S; Monici, M; Petersen, Ag; Jensen, Ub; Evert, K; Pantalone, D; Utpatel, K; Evert, M; Krüger, M; Wehland M.; Kopp, S; Frandsen, S; Corydon, T.; Sahana, J.; Bauer, J.; Lützenberg, R; Infanger, M.; Grimm, D.. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - ELETTRONICO. - 18:(2017), pp. 2604.2604-2604.2626. [10.3390/ijms18122604]
Preparation of a spaceflight: Apoptosis search in sutured wound healing models
Monici, MWriting – Original Draft Preparation
;Pantalone, DWriting – Review & Editing
;
2017
Abstract
To prepare the ESA (European Space Agency) spaceflight project “Wound healing and Sutures in Unloading Conditions”, we studied mechanisms of apoptosis in wound healing models based on ex vivo skin tissue cultures, kept for 10 days alive in serum-free DMEM/F12 medium supplemented with bovine serum albumin, hydrocortisone, insulin, ascorbic acid and antibiotics at 32 ◦C. The overall goal is to test: (i) the viability of tissue specimens; (ii) the gene expression of activators and inhibitors of apoptosis and extracellular matrix components in wound and suture models; and (iii) to design analytical protocols for future tissue specimens after post-spaceflight download.
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Descrizione: : To prepare the ESA (European Space Agency) spaceflight project “Wound healing and Sutures in Unloading Conditions”, we studied mechanisms of apoptosis in wound healing models based on ex vivo skin tissue cultures, kept for 10 days alive in serum-free DMEM/F12 medium supplemented with bovine serum albumin, hydrocortisone, insulin, ascorbic acid and antibiotics at 32
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