Abstract Purpose Circulating microRNAs (miRNA) have been described in patients with adrenocortical tumors, but the expression of miRNAs in non-functioning and cortisol-producing tumors has not been yet compared. Therefore, the objective of this study was to evaluate the expression of plasma extracellular vesicle (EV)-associated microRNAs in patients with non-functioning adrenocortical adenoma (NFA), cortisol-producing adrenocortical adenoma (CPA) and cortisol-producing adrenocortical carcinoma (CP-ACC). Methods Preoperative plasma EV samples of 13 NFAs, 13 CPAs and 9 CP-ACCs were subjected to extracellular vesicle isolation. miRNAs were investigated by targeted quantitative real-time PCR normalized to cel-miR-39 as reference. Five miRNAs have been selected for this analysis based on the previous studies including hsa-miR-22-3p, hsa-miR-27a-3p, hsamiR- 210-3p, hsa-miR-320b and hsa-miR-375. Results We have observed significant overrepresentation of three miRNAs in both CPA and CP-ACC relative to NFA: hsamiR- 22-3p (p o 0.01 and p o 0.0001, respectively), hsa-miR-27a-3p (p o 0.05 in both comparisons) and hsa-miR-320b (p o 0.05 and p o 0.0001, respectively). Hsa-miR-320b has been significantly overrepresented in CP-ACC relative to CPA (p o 0.01). Hsa-miR-210-3p turned out to be significantly overrepresented only in CP-ACC compared to NFA (p o 0.05). Significant correlation was revealed between circulating miRNA concentrations and urinary free cortisol values for hsa-miR- 22-3p, hsa-miR-27a-3p and hsa-miR-320b (p o 0.0001 for all) and cortisol after low-dose dexamethasone test for hsa-miR- 22-3p and hsa-miR-320b (p o 0.05). Hsa-miR-27a-3p has been significantly stimulated by low-dose dexamethasone test (p o 0.05). Conclusions EV-associated miRNAs are differentially expressed in different non-functioning and cortisol-producing adrenocortical tumors.

Analysis of circulating extracellular vesicle-associated microRNAs in cortisol-producing adrenocortical tumors / Perge, Pál; Decmann, Ábel; Pezzani, Raffaele; Bancos, Irina; Fassina, Ambrogio; Luconi, Michaela; Canu, Letizia; Tóth, Miklós; Boscaro, Marco; Patócs, Attila; Igaz, Peter. - In: ENDOCRINE. - ISSN 1355-008X. - ELETTRONICO. - 59:(2018), pp. 280-287. [10.1007/s12020-017-1506-z]

Analysis of circulating extracellular vesicle-associated microRNAs in cortisol-producing adrenocortical tumors

Luconi, Michaela;Canu, Letizia;
2018

Abstract

Abstract Purpose Circulating microRNAs (miRNA) have been described in patients with adrenocortical tumors, but the expression of miRNAs in non-functioning and cortisol-producing tumors has not been yet compared. Therefore, the objective of this study was to evaluate the expression of plasma extracellular vesicle (EV)-associated microRNAs in patients with non-functioning adrenocortical adenoma (NFA), cortisol-producing adrenocortical adenoma (CPA) and cortisol-producing adrenocortical carcinoma (CP-ACC). Methods Preoperative plasma EV samples of 13 NFAs, 13 CPAs and 9 CP-ACCs were subjected to extracellular vesicle isolation. miRNAs were investigated by targeted quantitative real-time PCR normalized to cel-miR-39 as reference. Five miRNAs have been selected for this analysis based on the previous studies including hsa-miR-22-3p, hsa-miR-27a-3p, hsamiR- 210-3p, hsa-miR-320b and hsa-miR-375. Results We have observed significant overrepresentation of three miRNAs in both CPA and CP-ACC relative to NFA: hsamiR- 22-3p (p o 0.01 and p o 0.0001, respectively), hsa-miR-27a-3p (p o 0.05 in both comparisons) and hsa-miR-320b (p o 0.05 and p o 0.0001, respectively). Hsa-miR-320b has been significantly overrepresented in CP-ACC relative to CPA (p o 0.01). Hsa-miR-210-3p turned out to be significantly overrepresented only in CP-ACC compared to NFA (p o 0.05). Significant correlation was revealed between circulating miRNA concentrations and urinary free cortisol values for hsa-miR- 22-3p, hsa-miR-27a-3p and hsa-miR-320b (p o 0.0001 for all) and cortisol after low-dose dexamethasone test for hsa-miR- 22-3p and hsa-miR-320b (p o 0.05). Hsa-miR-27a-3p has been significantly stimulated by low-dose dexamethasone test (p o 0.05). Conclusions EV-associated miRNAs are differentially expressed in different non-functioning and cortisol-producing adrenocortical tumors.
2018
59
280
287
Perge, Pál; Decmann, Ábel; Pezzani, Raffaele; Bancos, Irina; Fassina, Ambrogio; Luconi, Michaela; Canu, Letizia; Tóth, Miklós; Boscaro, Marco; Patócs, Attila; Igaz, Peter
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1111810
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