The networks of genes encoding palmitoylated proteins in axonal and synaptic compartments are affected in PPT1 overexpressing neuronal-like cells

The aim of this study was to recognize molecular signatures and functional modules connected with overexpressed CLN1 in human neuronal cellular system. We utilized SH-SY5Y neuroblastoma cells (differentiated into a neuronal-like phenotype) to overexpress wtCLN1 and a selection of disease related mutations previously detected in CLN1-affected children. The differentiated cell lines underwent whole transcriptomic profiling by RNA-seq, to identify differentially expressed genes (DEGs) involved in palmitoylation of neuronal proteins as well as related cellular functions.