We have studied two misfolded oligomeric forms of the protein HypF-N, which show similar morphologies but very different toxicities. We measured over 80 intermolecular distance-dependent parameters for each oligomer type using FRET, in conjunction with solution- and solid-state NMR and other biophysical techniques. The results indicate that the formation of a highly organised hydrogen bonded core in the toxic oligomers results in the exposure of a larger number of hydrophobic residues than in the nontoxic species, causing the former to form aberrant interactions with cellular components.

Structural differences between toxic and nontoxic HypF-N oligomers / Capitini, Claudia; Patel, Jayneil R.; Natalello, Antonino; D'Andrea, Cristiano; Relini, Annalisa; Jarvis, James A.; Birolo, Leila; Peduzzo, Alessia; Vendruscolo, Michele; Matteini, Paolo; Dobson, Christopher M.; De Simone, Alfonso; Chiti, Fabrizio*. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - STAMPA. - 54:(2018), pp. 8637-8640. [10.1039/c8cc03446j]

Structural differences between toxic and nontoxic HypF-N oligomers

CAPITINI, CLAUDIA;Chiti, Fabrizio
2018

Abstract

We have studied two misfolded oligomeric forms of the protein HypF-N, which show similar morphologies but very different toxicities. We measured over 80 intermolecular distance-dependent parameters for each oligomer type using FRET, in conjunction with solution- and solid-state NMR and other biophysical techniques. The results indicate that the formation of a highly organised hydrogen bonded core in the toxic oligomers results in the exposure of a larger number of hydrophobic residues than in the nontoxic species, causing the former to form aberrant interactions with cellular components.
2018
54
8637
8640
Capitini, Claudia; Patel, Jayneil R.; Natalello, Antonino; D'Andrea, Cristiano; Relini, Annalisa; Jarvis, James A.; Birolo, Leila; Peduzzo, Alessia; V...espandi
File in questo prodotto:
File Dimensione Formato  
Capitini et al. 2018.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 1.97 MB
Formato Adobe PDF
1.97 MB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1141619
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 25
social impact