FlowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possibly low concentrations. We here propose a dialysis-based modification of a commercial flowNMR setup that allows for recycling the medium while confining the sample (proteins and cells) within the active volume of the tube. This approach is demonstrated in the specific cases of in-cell NMR and protein-based ligand studies.
Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions / Cerofolini, Linda; Giuntini, Stefano; Barbieri, Letizia; Pennestri, Matteo; Codina, Anna; Fragai, Marco; Banci, Lucia; Luchinat, Enrico; Ravera, Enrico. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - ELETTRONICO. - 116:(2019), pp. 239-247. [10.1016/j.bpj.2018.11.3132]
Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
Cerofolini, Linda;Giuntini, Stefano;Barbieri, Letizia;Fragai, Marco;Banci, Lucia;Luchinat, Enrico;Ravera, Enrico
2019
Abstract
FlowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possibly low concentrations. We here propose a dialysis-based modification of a commercial flowNMR setup that allows for recycling the medium while confining the sample (proteins and cells) within the active volume of the tube. This approach is demonstrated in the specific cases of in-cell NMR and protein-based ligand studies.File | Dimensione | Formato | |
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