Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography.
Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics / Cerofolini, Linda; Giuntini, Stefano; Carlon, Azzurra; Ravera, Enrico; Calderone, Vito; Fragai, Marco; Parigi, Giacomo; Luchinat, Claudio. - In: CHEMISTRY-A EUROPEAN JOURNAL. - ISSN 0947-6539. - STAMPA. - 25:(2019), pp. 1-9. [10.1002/chem.201804488]
Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics
Cerofolini, Linda;Giuntini, Stefano;Carlon, Azzurra;Ravera, Enrico;Calderone, Vito;Fragai, Marco;Parigi, Giacomo;Luchinat, Claudio
2019
Abstract
Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography.File | Dimensione | Formato | |
---|---|---|---|
Asparaginase_Chemistry__A_European_Journal.pdf
Accesso chiuso
Tipologia:
Pdf editoriale (Version of record)
Licenza:
Tutti i diritti riservati
Dimensione
3.28 MB
Formato
Adobe PDF
|
3.28 MB | Adobe PDF | Richiedi una copia |
Chemistry_Eur_J_PEG_Asparaginase_2019.pdf
accesso aperto
Tipologia:
Versione finale referata (Postprint, Accepted manuscript)
Licenza:
Open Access
Dimensione
1.12 MB
Formato
Adobe PDF
|
1.12 MB | Adobe PDF |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.