Activation of plasma membrane H + -ATPases participates in dormancy alleviation in sunflower seeds

Using various inhibitors and scavengers we took advantage of the size of sunflower (Helianthus annuus) seeds to investigate in vivo the effects of hormones, namely abscisic acid (ABA) and ethylene (ET), and reactive oxygen species (ROS) on the polarization of dormant (D) and non-dormant (ND) embryonic seed cells using microelectrodes. Our data show that D and ND seed cells present different polarization likely due to the regulation of plasma membrane (PM) H⁺-ATPase activity. The data obtained after addition of hormones or ROS scavengers further suggest that ABA dependent inhibition of PM H⁺-ATPases could participate in dormancy maintenance and that ET-and ROS-dependent PM H⁺-ATPase stimulation could participate in dormancy release in sunflower seeds.