Expression of Phosphoinositide-Specific Phospholipase C Enzymes in Human Skin Fibroblasts

Fibroblasts are involved in a number of functions regulated by different signal transduction pathways, including the phosphoinositide (PI) signaling system and related converting enzymes, such as phosphoinositide-specific phospholipase C (PI-PLC). The PI-PLC family comprises crucial effector enzymes in the PI signal transduction pathway. Once activated, PI-PLC cleaves an important membrane PI, the phosphatidylinositol (4,5) bisphosphate into inositol trisphosphate and diacylglycerol-both are crucial molecules in the transduction of signals. The activity of selected PI-PLC enzymes was reported in fibroblasts, although the complete panel of expression was not available. Each cell type expresses a group of selected PI-PLC isoforms, and knowledge of the panel of expression is a necessary and preliminary tool to address further studies. In the present study, we delineated the expression panel of PI-PLC enzymes in human skin fibroblasts. PI-PLC beta 1, PI-PLC beta 3, PI-PLC beta 4, PI-PLC gamma 1, PI-PLC gamma 2, PI-PLC delta 1, PI-PLC delta 3, PI-PLC delta 4, and PI-PLC epsilon were expressed. PI-PLC beta 1 was weakly expressed, PI-PLC delta 4 was inconstantly expressed, and PI-PLC gamma 2 was weakly expressed.