Structural Biology of Iron-Binding Proteins by NMR Spectroscopy

Herein we provide an overview of the NMR strategies that have been designed in our lab to contribute answering some basic questions in inorganic structural biology, spanning from the initial exploitation of paramagnetic NMR constraints for the determination of the structural and dynamic properties of small isolated metalloproteins, to the study of ultra-weak protein-protein interaction up to the study of heme and iron trafficking. The reviewed examples address the role of some metalloproteins or metal-binding proteins in fundamental biological processes. The examples deal with macromolecular assemblies that are particularly challenging for NMR applications with respect to their complexity.