NMR measures a relatively small portion of the human metabolome, which roughly corresponds to all free small molecules present in concentrations ≥1 μM. However, the method is intrinsically quantitative and highly reproducible and, whenever standardized pre-analytical and analytical procedures are used, it allows for fast fingerprinting and profiling of a variety of biosamples. While developing methods for accurate and automated identification and quantitation of detectable metabolites, we have proposed a Urine Shift Predictor that also provides the concentration of NMR-invisible inorganic ions. For biomedical applications, two peculiar features are associated to the fast, untargeted NMR-metabolomic fingerprinting of biofluids, i.e. the ability to: i) identify the individual phenotype that constitutes the metabolic signature of a person and monitor its behavior over time; ii) identify the signature of different diseases. The combination of the two in longitudinal cohort studies could become a tool for fast and untargeted screening of populations.
Uniqueness of the NMR approach to metabolomics / Takis, Panteleimon G.; Ghini, Veronica; Tenori, Leonardo; Turano, Paola; Luchinat, Claudio. - In: TRAC. TRENDS IN ANALYTICAL CHEMISTRY. - ISSN 0165-9936. - ELETTRONICO. - 120:(2019), pp. 0-0. [10.1016/j.trac.2018.10.036]
Uniqueness of the NMR approach to metabolomics
Ghini, Veronica;Tenori, Leonardo;Turano, Paola
;Luchinat, Claudio
2019
Abstract
NMR measures a relatively small portion of the human metabolome, which roughly corresponds to all free small molecules present in concentrations ≥1 μM. However, the method is intrinsically quantitative and highly reproducible and, whenever standardized pre-analytical and analytical procedures are used, it allows for fast fingerprinting and profiling of a variety of biosamples. While developing methods for accurate and automated identification and quantitation of detectable metabolites, we have proposed a Urine Shift Predictor that also provides the concentration of NMR-invisible inorganic ions. For biomedical applications, two peculiar features are associated to the fast, untargeted NMR-metabolomic fingerprinting of biofluids, i.e. the ability to: i) identify the individual phenotype that constitutes the metabolic signature of a person and monitor its behavior over time; ii) identify the signature of different diseases. The combination of the two in longitudinal cohort studies could become a tool for fast and untargeted screening of populations.File | Dimensione | Formato | |
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