In this work, an electrochemical enzyme-linked oligonucleotide array to achieve simple and rapid multidetection of aflatoxin B 1 (AFB 1 ) is presented. The assay is based on a competitive format and disposable screen-printed cells (SPCs). Firstly, the electrodeposition of poly(aniline-anthranilic acid) copolymer (PANI-PAA) on graphite screen-printed working electrodes was performed by means of cyclic voltammetry (CV). Aflatoxin B 1 conjugated with bovine serum albumin (AFB 1 -BSA) was then immobilized by covalent binding on PANI-PAA copolymer. After performing the affinity reaction between AFB 1 and the biotinylated DNA-aptamer (apt-BIO), the solution was dropped on the modified SPCs and the competition was carried out. The biotinylated complexes formed onto the sensor surface were coupled with a streptavidin-alkaline phosphatase conjugate. 1-naphthyl phosphate was used as enzymatic substrate; the electroactive product was detected by differential pulse voltammetry (DPV). The response of the enzyme-linked oligonucleotide assay was signal-off, according to the competitive format. A dose-response curve was obtained between 0.1 ng mL −1 and 10 ng mL −1 and a limit of detection of 0.086 ng mL −1 was achieved. Finally, preliminary experiments in maize flour samples spiked with AFB 1 were also performed.

Electrochemical enzyme-linked oligonucleotide array for aflatoxin B 1 detection / Selvolini, Giulia; Lettieri, Mariagrazia; Tassoni, Luca; Gastaldello, Silvia; Grillo, Maria; Maran, Claudio; Marrazza, Giovanna. - In: TALANTA. - ISSN 0039-9140. - STAMPA. - 203:(2019), pp. 49-57. [10.1016/j.talanta.2019.05.044]

Electrochemical enzyme-linked oligonucleotide array for aflatoxin B 1 detection

Selvolini, Giulia;LETTIERI, MARIAGRAZIA;Marrazza, Giovanna
2019

Abstract

In this work, an electrochemical enzyme-linked oligonucleotide array to achieve simple and rapid multidetection of aflatoxin B 1 (AFB 1 ) is presented. The assay is based on a competitive format and disposable screen-printed cells (SPCs). Firstly, the electrodeposition of poly(aniline-anthranilic acid) copolymer (PANI-PAA) on graphite screen-printed working electrodes was performed by means of cyclic voltammetry (CV). Aflatoxin B 1 conjugated with bovine serum albumin (AFB 1 -BSA) was then immobilized by covalent binding on PANI-PAA copolymer. After performing the affinity reaction between AFB 1 and the biotinylated DNA-aptamer (apt-BIO), the solution was dropped on the modified SPCs and the competition was carried out. The biotinylated complexes formed onto the sensor surface were coupled with a streptavidin-alkaline phosphatase conjugate. 1-naphthyl phosphate was used as enzymatic substrate; the electroactive product was detected by differential pulse voltammetry (DPV). The response of the enzyme-linked oligonucleotide assay was signal-off, according to the competitive format. A dose-response curve was obtained between 0.1 ng mL −1 and 10 ng mL −1 and a limit of detection of 0.086 ng mL −1 was achieved. Finally, preliminary experiments in maize flour samples spiked with AFB 1 were also performed.
2019
203
49
57
Selvolini, Giulia; Lettieri, Mariagrazia; Tassoni, Luca; Gastaldello, Silvia; Grillo, Maria; Maran, Claudio; Marrazza, Giovanna
File in questo prodotto:
File Dimensione Formato  
Talanta_2019_B.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 5.21 MB
Formato Adobe PDF
5.21 MB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1156954
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 61
  • ???jsp.display-item.citation.isi??? 54
social impact