Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two synthetic carbohydrate antigens related to serotypes 19F and 14 of Streptococcus pneumoniae and evaluated their immunogenicity in vivo. A tetrasaccharide fragment of serotype 14 (Tetra-14), a trisaccharide fragment of serotype 19F (Tri-19F), a T-helper peptide and D-glucose were loaded onto GNP in different ratios. Mice immunization showed that the concomitant presence of Tri-19F and Tetra-14 on the same nanoparticle critically enhanced the titers of specific IgG antibodies toward type 14 polysaccharide compared with GNP exclusively displaying Tetra-14, while no IgG antibodies against type 19F polysaccharide were elicited. This work is a step forward toward synthetic nanosystems combining synthetic carbohydrate antigens and immunogenic peptides as potential carbohydrate-based vaccines.
Preparation and immunogenicity of gold glyco-nanoparticles as antipneumococcal vaccine model / Vetro, Maria; Safari, Dodi; Fallarini, Silvia; Salsabila, Korrie; Lahmann, Martina; Penadés, Soledad; Lay, Luigi; Marradi, Marco; Compostella, Federica. - In: NANOMEDICINE. - ISSN 1743-5889. - ELETTRONICO. - 12:(2017), pp. 13-23. [10.2217/nnm-2016-0306]
Preparation and immunogenicity of gold glyco-nanoparticles as antipneumococcal vaccine model
Marradi, Marco;
2017
Abstract
Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two synthetic carbohydrate antigens related to serotypes 19F and 14 of Streptococcus pneumoniae and evaluated their immunogenicity in vivo. A tetrasaccharide fragment of serotype 14 (Tetra-14), a trisaccharide fragment of serotype 19F (Tri-19F), a T-helper peptide and D-glucose were loaded onto GNP in different ratios. Mice immunization showed that the concomitant presence of Tri-19F and Tetra-14 on the same nanoparticle critically enhanced the titers of specific IgG antibodies toward type 14 polysaccharide compared with GNP exclusively displaying Tetra-14, while no IgG antibodies against type 19F polysaccharide were elicited. This work is a step forward toward synthetic nanosystems combining synthetic carbohydrate antigens and immunogenic peptides as potential carbohydrate-based vaccines.File | Dimensione | Formato | |
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Nanomedicine+2017.pdf
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