The development of tools for the early diagnosis of pancreatic adenocarcinoma is an urgent need in order to increase treatment success rate and reduce patient mortality. Here, we present a modular nanosystem platform integrating soft nanoparticles with a targeting peptide and an active imaging agent for diagnostics. Biocompatible single-chain polymer nanoparticles (SCPNs) based on poly(methacrylic acid) were prepared and functionalized with the somatostatin analogue PTR86 as the targeting moiety, since somatostatin receptors are overexpressed in pancreatic cancer. The gamma emitter 67Ga was incorporated by chelation and allowed in vivo investigation of the pharmacokinetic properties of the nanoparticles using single photon emission computerized tomography (SPECT). The resulting engineered nanosystem was tested in a xenograph mouse model of human pancreatic adenocarcinoma. Imaging results demonstrate that accumulation of targeted SCPNs in the tumor is higher than that observed for nontargeted nanoparticles due to improved retention in this tissue.

Functional Single-Chain Polymer Nanoparticles: Targeting and Imaging Pancreatic Tumors in Vivo / Benito, Ana B; Aiertza, Miren K; Marradi, Marco; Gil-Iceta, Larraitz; Shekhter Zahavi, Talia; Szczupak, Boguslaw; Jiménez-González, María; Reese, Torsten; Scanziani, Eugenio; Passoni, Lorena; Matteoli, Michela; De Maglie, Marcella; Orenstein, Arie; Oron-Herman, Mor; Kostenich, Gennady; Buzhansky, Ludmila; Gazit, Ehud; Grande, Hans-Jurgen; Gómez-Vallejo, Vanessa; Llop, Jordi; Loinaz, Iraida. - In: BIOMACROMOLECULES. - ISSN 1525-7797. - ELETTRONICO. - 17:(2016), pp. 3213-3221. [10.1021/acs.biomac.6b00941]

Functional Single-Chain Polymer Nanoparticles: Targeting and Imaging Pancreatic Tumors in Vivo

Marradi, Marco;
2016

Abstract

The development of tools for the early diagnosis of pancreatic adenocarcinoma is an urgent need in order to increase treatment success rate and reduce patient mortality. Here, we present a modular nanosystem platform integrating soft nanoparticles with a targeting peptide and an active imaging agent for diagnostics. Biocompatible single-chain polymer nanoparticles (SCPNs) based on poly(methacrylic acid) were prepared and functionalized with the somatostatin analogue PTR86 as the targeting moiety, since somatostatin receptors are overexpressed in pancreatic cancer. The gamma emitter 67Ga was incorporated by chelation and allowed in vivo investigation of the pharmacokinetic properties of the nanoparticles using single photon emission computerized tomography (SPECT). The resulting engineered nanosystem was tested in a xenograph mouse model of human pancreatic adenocarcinoma. Imaging results demonstrate that accumulation of targeted SCPNs in the tumor is higher than that observed for nontargeted nanoparticles due to improved retention in this tissue.
2016
17
3213
3221
Benito, Ana B; Aiertza, Miren K; Marradi, Marco; Gil-Iceta, Larraitz; Shekhter Zahavi, Talia; Szczupak, Boguslaw; Jiménez-González, María; Reese, Torsten; Scanziani, Eugenio; Passoni, Lorena; Matteoli, Michela; De Maglie, Marcella; Orenstein, Arie; Oron-Herman, Mor; Kostenich, Gennady; Buzhansky, Ludmila; Gazit, Ehud; Grande, Hans-Jurgen; Gómez-Vallejo, Vanessa; Llop, Jordi; Loinaz, Iraida
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1157154
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