Purpose We evaluated the effect of low-calorie mediterranean (MD) and vegetarian (VD) diets on gut microbiome (GM) composition and short-chain-fatty acids (SCFA) production. Methods We performed next generation sequencing (NGS) of 16S rRNA and SCFA analysis on fecal samples of 23 overweight omnivores (16 F; 7 M) with low-to-moderate cardiovascular risk. They were randomly assigned to a VD or MD, each lasting 3 months, with a crossover study design. Results Dietary interventions did not produce significant diversity in the GM composition at higher ranks (family and above), neither between nor within MD and VD, but they did it at genus level. MD significantly changed the abundance of Enterorhabdus, Lachnoclostridium and Parabacteroides, while VD significantly affected the abundance of Anaerostipes, Streptococcus, Clostridium sensu stricto, and Odoribacter. Comparison of the mean variation of each SCFA between MD and VD showed an opposite and statistically significant trend for propionic acid (+ 10% vs − 28%, respectively, p = 0.034). In addition, variations of SCFA were negatively correlated with changes of some inflammatory cytokines such as VEGF, MCP-1, IL-17, IP-10 and IL-12, only after MD. Finally, correlation analyses showed a potential relationship—modulated by the two diets—between changes of genera and changes of clinical and biochemical parameters. Conclusions A short-term dietary intervention with MD or VD does not induce major change in the GM, suggesting that a diet should last longer than 3 months for scratching the microbial resilience. Changes in SCFA production support their role in modulating the inflammatory response, thus mediating the anti-inflammatory and protective properties of MD.

Influence of a 3-month low-calorie Mediterranean diet compared to the vegetarian diet on human gut microbiota and SCFA: The CARDIVEG study / Pagliai, Giuditta; Russo, Edda; Niccolai, Elena; Dinu, Monica; Di Pilato, Vincenzo; Magrini, Alessandro; Bartolucci, Gianluca; Baldi, Simone; Menicatti, Marta; Giusti, Betti; Marcucci, Rossella; Rossolini, Gian Maria; Casini, Alessandro; Sofi, Francesco; Amedei, Amedeo. - In: EUROPEAN JOURNAL OF NUTRITION. - ISSN 1436-6207. - ELETTRONICO. - 59:(2019), pp. 2011-2024. [10.1007/s00394-019-02050-0]

Influence of a 3-month low-calorie Mediterranean diet compared to the vegetarian diet on human gut microbiota and SCFA: The CARDIVEG study

Pagliai, Giuditta
Writing – Original Draft Preparation
;
Russo, Edda
Writing – Original Draft Preparation
;
Niccolai, Elena
Formal Analysis
;
Dinu, Monica
Formal Analysis
;
Di Pilato, Vincenzo
Formal Analysis
;
Magrini, Alessandro
Investigation
;
Bartolucci, Gianluca;Baldi, Simone;Menicatti, Marta
Formal Analysis
;
Giusti, Betti
Writing – Review & Editing
;
Marcucci, Rossella
Writing – Review & Editing
;
Rossolini, Gian Maria
Formal Analysis
;
Casini, Alessandro
Writing – Review & Editing
;
Sofi, Francesco
Supervision
;
Amedei, Amedeo
Supervision
2019

Abstract

Purpose We evaluated the effect of low-calorie mediterranean (MD) and vegetarian (VD) diets on gut microbiome (GM) composition and short-chain-fatty acids (SCFA) production. Methods We performed next generation sequencing (NGS) of 16S rRNA and SCFA analysis on fecal samples of 23 overweight omnivores (16 F; 7 M) with low-to-moderate cardiovascular risk. They were randomly assigned to a VD or MD, each lasting 3 months, with a crossover study design. Results Dietary interventions did not produce significant diversity in the GM composition at higher ranks (family and above), neither between nor within MD and VD, but they did it at genus level. MD significantly changed the abundance of Enterorhabdus, Lachnoclostridium and Parabacteroides, while VD significantly affected the abundance of Anaerostipes, Streptococcus, Clostridium sensu stricto, and Odoribacter. Comparison of the mean variation of each SCFA between MD and VD showed an opposite and statistically significant trend for propionic acid (+ 10% vs − 28%, respectively, p = 0.034). In addition, variations of SCFA were negatively correlated with changes of some inflammatory cytokines such as VEGF, MCP-1, IL-17, IP-10 and IL-12, only after MD. Finally, correlation analyses showed a potential relationship—modulated by the two diets—between changes of genera and changes of clinical and biochemical parameters. Conclusions A short-term dietary intervention with MD or VD does not induce major change in the GM, suggesting that a diet should last longer than 3 months for scratching the microbial resilience. Changes in SCFA production support their role in modulating the inflammatory response, thus mediating the anti-inflammatory and protective properties of MD.
2019
59
2011
2024
Goal 3: Good health and well-being for people
Pagliai, Giuditta; Russo, Edda; Niccolai, Elena; Dinu, Monica; Di Pilato, Vincenzo; Magrini, Alessandro; Bartolucci, Gianluca; Baldi, Simone; Menicatti, Marta; Giusti, Betti; Marcucci, Rossella; Rossolini, Gian Maria; Casini, Alessandro; Sofi, Francesco; Amedei, Amedeo
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1161501
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