Antibiotics released in the environment exert a selective pressure on the resident microbiota. It is well accepted that the mere measurement of antibiotics does not reflect the actual bioavailability. In fact, antibiotics can be adsorbed or complexed to particles and/or chemicals in water and soil. Bioavailable concentrations of antibiotics in soil and water are subjected to great uncertainty, therefore biological assays are increasingly recognized as that allow an indirect determination of the residual antibiotic activity. Here we propose how a fitness test for bacteria can be used to qualitatively assess the bioavailability of a specific antibiotic in the environment. The findings show that by using a pair of resistant and sensitive bacterial strains, the resulting fitness can indirectly reflect antibiotic bioavailability. Hence, this test can be used as a complementary assay to other biological and chemical tests to assess bioavailability of antibiotics.

A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test / Massimiliano Marvasi, Brunella Perito, Alessandro Canali, Ajit Shah, Vlad Serafim. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - STAMPA. - (2019), pp. 0-0.

A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test

Massimiliano Marvasi
;
Brunella Perito;
2019

Abstract

Antibiotics released in the environment exert a selective pressure on the resident microbiota. It is well accepted that the mere measurement of antibiotics does not reflect the actual bioavailability. In fact, antibiotics can be adsorbed or complexed to particles and/or chemicals in water and soil. Bioavailable concentrations of antibiotics in soil and water are subjected to great uncertainty, therefore biological assays are increasingly recognized as that allow an indirect determination of the residual antibiotic activity. Here we propose how a fitness test for bacteria can be used to qualitatively assess the bioavailability of a specific antibiotic in the environment. The findings show that by using a pair of resistant and sensitive bacterial strains, the resulting fitness can indirectly reflect antibiotic bioavailability. Hence, this test can be used as a complementary assay to other biological and chemical tests to assess bioavailability of antibiotics.
2019
0
0
Massimiliano Marvasi, Brunella Perito, Alessandro Canali, Ajit Shah, Vlad Serafim
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1171534
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