Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible in protein solid-state NMR. We present the first ultra-high dimensional implementation of this approach where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular size and aggregation state, featuring limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis, yielding rapid and unbiased backbone resonance assignments.

Protein NMR resonance assignment without spectral analysis: 5D SOlid‐state Automated Projection SpectroscopY (SO‐APSY) / Orton, Henry W.; Stanek, Jan; Schubeis, Tobias; Foucaudeau, Dylan; Ollier, Claire; Draney, Adrian W.; Le Marchand, Tanguy; De Paepe, Diane Cala; Felli, Isabella C.; Pierattelli, Roberta; Hiller, Sebastian; Bermel, Wolfgang; Pintacuda, Guido. - In: ANGEWANDTE CHEMIE. - ISSN 0044-8249. - STAMPA. - 59:(2020), pp. 2380-2384. [10.1002/anie.201912211]

Protein NMR resonance assignment without spectral analysis: 5D SOlid‐state Automated Projection SpectroscopY (SO‐APSY)

Felli, Isabella C.;Pierattelli, Roberta;
2020

Abstract

Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible in protein solid-state NMR. We present the first ultra-high dimensional implementation of this approach where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular size and aggregation state, featuring limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis, yielding rapid and unbiased backbone resonance assignments.
2020
59
2380
2384
Orton, Henry W.; Stanek, Jan; Schubeis, Tobias; Foucaudeau, Dylan; Ollier, Claire; Draney, Adrian W.; Le Marchand, Tanguy; De Paepe, Diane Cala; Felli, Isabella C.; Pierattelli, Roberta; Hiller, Sebastian; Bermel, Wolfgang; Pintacuda, Guido
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1175042
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