Aims: The objective of this study was to investigate the possible synergy between doxycycline and photodynamic therapy (PDT) against Helicobacter pylori and to evaluate the possible side effects on gastric cells (AGS cell line) with and without protoporphyrin IX. Materials & Methods: Three H. pylori strains (ATCC 700392, 43504 and 49503), were grown on solid medium either containing or not doxycycline at sub-inhibitory concentrations, and irradiated for 10, 20 and 30 minutes with a 400 nm-peaked light source. The phototoxicity tests on AGS cells was evaluated by MTT assay. Results : The PDT and doxycycline combination showed an antibacterial synergistic effect with no significant toxicities. Conclusions: The synergistic treatment could be considered as an interesting therapeutic option.

Synergistic effect of photodynamic therapy at 400 nm and doxycycline against Helicobacter pylori / Ilaria Baccani, Paola Faraoni, Matilde Marini, Alessio Gnerucci, Barbara Orsini, Patrizia Pecile, Giovanni Romano, Franco Fusi, Gian Maria Rossolini, Alberto Antonelli. - In: FUTURE MICROBIOLOGY. - ISSN 1746-0913. - ELETTRONICO. - 14:(2019), pp. 1199-1205. [10.2217/fmb-2019-0129]

Synergistic effect of photodynamic therapy at 400 nm and doxycycline against Helicobacter pylori

Ilaria Baccani
Investigation
;
Paola Faraoni
Writing – Original Draft Preparation
;
Matilde Marini
Investigation
;
Alessio Gnerucci
Formal Analysis
;
Barbara Orsini
Methodology
;
Patrizia Pecile
Methodology
;
Giovanni Romano
Supervision
;
Franco Fusi
Conceptualization
;
Gian Maria Rossolini
Writing – Review & Editing
;
Alberto Antonelli
Investigation
2019

Abstract

Aims: The objective of this study was to investigate the possible synergy between doxycycline and photodynamic therapy (PDT) against Helicobacter pylori and to evaluate the possible side effects on gastric cells (AGS cell line) with and without protoporphyrin IX. Materials & Methods: Three H. pylori strains (ATCC 700392, 43504 and 49503), were grown on solid medium either containing or not doxycycline at sub-inhibitory concentrations, and irradiated for 10, 20 and 30 minutes with a 400 nm-peaked light source. The phototoxicity tests on AGS cells was evaluated by MTT assay. Results : The PDT and doxycycline combination showed an antibacterial synergistic effect with no significant toxicities. Conclusions: The synergistic treatment could be considered as an interesting therapeutic option.
2019
14
1199
1205
Ilaria Baccani, Paola Faraoni, Matilde Marini, Alessio Gnerucci, Barbara Orsini, Patrizia Pecile, Giovanni Romano, Franco Fusi, Gian Maria Rossolini, Alberto Antonelli
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1176555
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