In cancer patients, circulating cell-free DNA (ccfDNA) can contain tumor-derived DNA (ctDNA), which enables noninvasive diagnosis, real-time monitoring, and treatment susceptibility testing. However, ctDNA fractions are highly variable, which challenges downstream applications. Therefore, established preanalytical work flows in combination with cost-efficient and reproducible reference materials for ccfDNA analyses are crucial for analytical validity and subsequently for clinical decision-making.
Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of Standardized (Pre)analytical Work Flows / Lampignano, Rita; Neumann, Martin H D; Weber, Sabrina; Kloten, Vera; Herdean, Andrei; Voss, Thorsten; Groelz, Daniel; Babayan, Anna; Tibbesma, Marco; Schlumpberger, Martin; Chemi, Francesca; Rothwell, Dominic G; Wikman, Harriet; Galizzi, Jean-Pierre; Bergheim, Inger Riise; Russnes, Hege; Mussolin, Benedetta; Bonin, Serena; Voigt, Christine; Musa, Hanny; Pinzani, Pamela; Lianidou, Evi; Brady, Ged; Speicher, Michael R; Pantel, Klaus; Betsou, Fay; Schuuring, Ed; Kubista, Mikael; Ammerlaan, Wim; Sprenger-Haussels, Markus; Schlange, Thomas; Heitzer, Ellen. - In: CLINICAL CHEMISTRY. - ISSN 0009-9147. - STAMPA. - 66:(2019), pp. 149-160. [10.1373/clinchem.2019.306837]
Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of Standardized (Pre)analytical Work Flows
Pinzani, PamelaMembro del Collaboration Group
;
2019
Abstract
In cancer patients, circulating cell-free DNA (ccfDNA) can contain tumor-derived DNA (ctDNA), which enables noninvasive diagnosis, real-time monitoring, and treatment susceptibility testing. However, ctDNA fractions are highly variable, which challenges downstream applications. Therefore, established preanalytical work flows in combination with cost-efficient and reproducible reference materials for ccfDNA analyses are crucial for analytical validity and subsequently for clinical decision-making.
| File | Dimensione | Formato | |
|---|---|---|---|
|
clinchem.2019.306837 Cancer Id.pdf
accesso aperto
Tipologia:
Pdf editoriale (Version of record)
Licenza:
Open Access
Dimensione
2.48 MB
Formato
Adobe PDF
|
2.48 MB | Adobe PDF |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
