Background: Neuromuscular transmission has been extensively studied in the cir-cular layer of the mouse colon where a co-transmission of purines acting on P2Y1receptors and NO has been previously described. However, the corresponding mech-anisms in the longitudinal layer are less known.Methods: Electrophysiological and myography techniques were used to evaluate spontaneous phasic contractions (SPC) and neural-mediated responses in the proxi-mal, mid, and distal colon devoid of CD1 mice. Immunohistochemistry against c-kit and PDGFRα was performed in each colonic segment.Key Results: SPC were recorded in both muscle layers at a similar frequency being about four contractions per minute (c.p.m.) in the proximal and distal colon compared to the mid colon (2 c.p.m.). In non-adrenergic, non-cholinergic conditions, L-NNA (1 mmol/L) increased contractility in the circular but not in the longitudinal layer. In the longitudinal muscle, both electrophysiological and mechanical neural-mediated inhibitory responses were L-NNA and ODQ (10 μmol/L) sensitive. NaNP (1 μmol/L) caused cessation of SPC and the response was blocked by ODQ. Neither ADPßS (10 μmol/L) nor CYPPA (10 μmol/L), which both targeted the purinergic pathway, altered longitudinal contractions. PDGFRα + cells were located in both muscle layers and were more numerous compared with cKit + cells, which both formed a heterolo-gous cellular network. A decreasing gradient of the PDGFRα labeling was observed along the colon.Conclusion: An inhibitory neural tone was absent in the longitudinal layer and neuronal inhibitory responses were mainly nitrergic. Despite the presence of PDGFRα + cells, purinergic responses were absent. Post-junctional pathways located in different cell types might be responsible for neurotransmitter transduction.

The asymmetric innervation of the circular and longitudinal muscle of the mouse colon differently modulates myogenic slow phasic contractions / Sara Traserra, Sonia Villarte, Chiara Traini, Sara Palacin, Patri Vergara, Maria Giuliana Vannucchi, Marcel Jimenez. - In: NEUROGASTROENTEROLOGY & MOTILITY. - ISSN 1365-2982. - ELETTRONICO. - ...:(2020), pp. 0-0.

The asymmetric innervation of the circular and longitudinal muscle of the mouse colon differently modulates myogenic slow phasic contractions.

Chiara Traini;Maria Giuliana Vannucchi;
2020

Abstract

Background: Neuromuscular transmission has been extensively studied in the cir-cular layer of the mouse colon where a co-transmission of purines acting on P2Y1receptors and NO has been previously described. However, the corresponding mech-anisms in the longitudinal layer are less known.Methods: Electrophysiological and myography techniques were used to evaluate spontaneous phasic contractions (SPC) and neural-mediated responses in the proxi-mal, mid, and distal colon devoid of CD1 mice. Immunohistochemistry against c-kit and PDGFRα was performed in each colonic segment.Key Results: SPC were recorded in both muscle layers at a similar frequency being about four contractions per minute (c.p.m.) in the proximal and distal colon compared to the mid colon (2 c.p.m.). In non-adrenergic, non-cholinergic conditions, L-NNA (1 mmol/L) increased contractility in the circular but not in the longitudinal layer. In the longitudinal muscle, both electrophysiological and mechanical neural-mediated inhibitory responses were L-NNA and ODQ (10 μmol/L) sensitive. NaNP (1 μmol/L) caused cessation of SPC and the response was blocked by ODQ. Neither ADPßS (10 μmol/L) nor CYPPA (10 μmol/L), which both targeted the purinergic pathway, altered longitudinal contractions. PDGFRα + cells were located in both muscle layers and were more numerous compared with cKit + cells, which both formed a heterolo-gous cellular network. A decreasing gradient of the PDGFRα labeling was observed along the colon.Conclusion: An inhibitory neural tone was absent in the longitudinal layer and neuronal inhibitory responses were mainly nitrergic. Despite the presence of PDGFRα + cells, purinergic responses were absent. Post-junctional pathways located in different cell types might be responsible for neurotransmitter transduction.
2020
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Goal 3: Good health and well-being for people
Sara Traserra, Sonia Villarte, Chiara Traini, Sara Palacin, Patri Vergara, Maria Giuliana Vannucchi, Marcel Jimenez
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1181437
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