The relevance of extracellular DNA (eDNA) in the soil ecosystem is becoming more and more evident to the scientific community by the progressive discovery of functions accompanying to natural gene transformation. However, despite the increased number of published articles dedicated to eDNA in soil, so far only few are focused on its single stranded form (eDNAss). The present paper is the first to investigate the quantitative relevance of eDNAss in the total soil eDNA pool, discriminating between its linear (eDNAssl) and circular (eDNAssc) forms and the respective weakly (wa) and tightly (ta) adsorbed fractions. The results showed the prevalence of eDNAss and its linear form in both the total soil eDNA pool and its wa and ta fractions. Both of the eDNAss fractions (linear and circular) were characterized by small fragments.

Preliminary evidences of the presence of extracellular DNA single stranded forms in soil / Pathan, Shamina Imran; Arfaioli, Paola; Ceccherini, Maria Teresa; Ascher-Jenull, Judith; Pietramellara, Giacomo. - In: PLOS ONE. - ISSN 1932-6203. - ELETTRONICO. - 15 - Issue (1):(2020), pp. 1-10. [10.1371/journal.pone.0227296]

Preliminary evidences of the presence of extracellular DNA single stranded forms in soil

Pathan, Shamina Imran;Arfaioli, Paola;Ceccherini, Maria Teresa;Pietramellara, Giacomo
2020

Abstract

The relevance of extracellular DNA (eDNA) in the soil ecosystem is becoming more and more evident to the scientific community by the progressive discovery of functions accompanying to natural gene transformation. However, despite the increased number of published articles dedicated to eDNA in soil, so far only few are focused on its single stranded form (eDNAss). The present paper is the first to investigate the quantitative relevance of eDNAss in the total soil eDNA pool, discriminating between its linear (eDNAssl) and circular (eDNAssc) forms and the respective weakly (wa) and tightly (ta) adsorbed fractions. The results showed the prevalence of eDNAss and its linear form in both the total soil eDNA pool and its wa and ta fractions. Both of the eDNAss fractions (linear and circular) were characterized by small fragments.
2020
15 - Issue (1)
1
10
Pathan, Shamina Imran; Arfaioli, Paola; Ceccherini, Maria Teresa; Ascher-Jenull, Judith; Pietramellara, Giacomo
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1181588
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