In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward the center of the sarcomere, the structural unit of striated muscle. Cross-bridge attachments in the sarcomere have been reported to exhibit a similar stiffness under both positive and negative forces. However, in vitro measurements on filaments with a sparse complement of heads detected a decrease of the cross-bridge stiffness at negative forces attributed to the buckling of the subfragment 2 tail portion. Here, we review some old and new data that confirm that cross-bridge stiffness is nearly linear in the muscle filament lattice. The implications of high myosin stiffness at positive and negative strains are considered in muscle fibers and in nonmuscle intracellular cargo transport.

Straightening Out the Elasticity of Myosin Cross-Bridges / Linari M.; Piazzesi G.; Pertici I.; Dantzig J.A.; Goldman Y.E.; Lombardi V.. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - STAMPA. - 118:(2020), pp. 994-1002. [10.1016/j.bpj.2020.01.002]

Straightening Out the Elasticity of Myosin Cross-Bridges

Linari M.
Conceptualization
;
Piazzesi G.
Conceptualization
;
Pertici I.
Data Curation
;
2020

Abstract

In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward the center of the sarcomere, the structural unit of striated muscle. Cross-bridge attachments in the sarcomere have been reported to exhibit a similar stiffness under both positive and negative forces. However, in vitro measurements on filaments with a sparse complement of heads detected a decrease of the cross-bridge stiffness at negative forces attributed to the buckling of the subfragment 2 tail portion. Here, we review some old and new data that confirm that cross-bridge stiffness is nearly linear in the muscle filament lattice. The implications of high myosin stiffness at positive and negative strains are considered in muscle fibers and in nonmuscle intracellular cargo transport.
2020
118
994
1002
Linari M.; Piazzesi G.; Pertici I.; Dantzig J.A.; Goldman Y.E.; Lombardi V.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1192240
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