AIMS: We investigated the role played by serotonin (5-HT) receptors within the preBötzinger complex (preBötC), the Bötzinger complex and the rostral inspiratory ventral respiratory group in the modulation of respiratory activity. METHODS: Experiments were performed on α-chloralose-urethane anesthetized, vagotomized, paralyzed and artificially ventilated rabbits making use of neuronal recordings and bilateral microinjections (30-50 nl). The presence of GABAergic neurons and 5-HT1A receptors in the preBötC region was investigated by immunohistochemistry.RESULTS: 5-HT caused excitatory effects on respiratory activity only when applied to the preBötC region. These effects were mediated only by 5-HT1A and 5-HT3 receptors as shown by microinjections of specific agonists of the different types of serotonin receptors, including 8-OH-DPAT or BP 554 and phenylbiguanide. Unexpectedly, the blockade of 5-HT1A receptors by methysergide or the specific antagonist (S)-WAY 100135 induced excitatory respiratory effects. Microinjections of the 5-HT3 receptor antagonist ondansetron did not influence respiration, but prevented (S)-WAY 100135-induced responses. The blockade of GABAA receptors by bicuculline within the preBötC prevented the effects of the 5-HT1A receptor agonist 8-OH-DPAT. The involvement of GABAergic inhibition and 5-HT1A receptor-mediated disinhibition is also corroborated by immunohistochemical data. CONCLUSIONS: The results show for the first time in an adult animal preparation that 5-HT plays a pivotal role in the modulation of the preBötC activity through an action on 5-HT1A and 5-HT3 receptors probably both at pre- and postsynaptic level and highlight the importance of disinhibition phenomena. Present findings could be relevant to some respiratory disorders.

Excitatory effects on respiratory activity mediated by 5-HT1A and 5-HT3 receptors within the preBötzinger Complex of the adult rabbit / Cinelli E., L. Iovino, D. Mutolo, T. Pantaleo, F. Bongianni.. - ELETTRONICO. - (2018), pp. 1-1. (Intervento presentato al convegno 11th Forum of Neuroscience tenutosi a Berlino nel 7-11 Luglio 2018).

Excitatory effects on respiratory activity mediated by 5-HT1A and 5-HT3 receptors within the preBötzinger Complex of the adult rabbit

Cinelli E.;L. Iovino;D. Mutolo;T. Pantaleo;F. Bongianni.
2018

Abstract

AIMS: We investigated the role played by serotonin (5-HT) receptors within the preBötzinger complex (preBötC), the Bötzinger complex and the rostral inspiratory ventral respiratory group in the modulation of respiratory activity. METHODS: Experiments were performed on α-chloralose-urethane anesthetized, vagotomized, paralyzed and artificially ventilated rabbits making use of neuronal recordings and bilateral microinjections (30-50 nl). The presence of GABAergic neurons and 5-HT1A receptors in the preBötC region was investigated by immunohistochemistry.RESULTS: 5-HT caused excitatory effects on respiratory activity only when applied to the preBötC region. These effects were mediated only by 5-HT1A and 5-HT3 receptors as shown by microinjections of specific agonists of the different types of serotonin receptors, including 8-OH-DPAT or BP 554 and phenylbiguanide. Unexpectedly, the blockade of 5-HT1A receptors by methysergide or the specific antagonist (S)-WAY 100135 induced excitatory respiratory effects. Microinjections of the 5-HT3 receptor antagonist ondansetron did not influence respiration, but prevented (S)-WAY 100135-induced responses. The blockade of GABAA receptors by bicuculline within the preBötC prevented the effects of the 5-HT1A receptor agonist 8-OH-DPAT. The involvement of GABAergic inhibition and 5-HT1A receptor-mediated disinhibition is also corroborated by immunohistochemical data. CONCLUSIONS: The results show for the first time in an adult animal preparation that 5-HT plays a pivotal role in the modulation of the preBötC activity through an action on 5-HT1A and 5-HT3 receptors probably both at pre- and postsynaptic level and highlight the importance of disinhibition phenomena. Present findings could be relevant to some respiratory disorders.
2018
11th Forum of Neuroscience, Berlin, 7-11 July 2018
11th Forum of Neuroscience
Berlino
Goal 3: Good health and well-being for people
Cinelli E., L. Iovino, D. Mutolo, T. Pantaleo, F. Bongianni.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1196415
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