The current paradigm for protein structure determination by NMR spectroscopy has thus far been based on collecting distance restrains between pairs of nuclei in the form of nuclear Overhauser enhancements (NOE). These, have in some circumstances been supplemented with other information sources such as paramagnetic relaxation enhancements or residual dipolar couplings. Here, we report for the first time a protein structure determination by NMR without the use of NOEs. The protein PioC from Rhodopseu-domonas palustris TIE-1 is an HIPIP where the 4Fe4S cluster is paramagnetic and provides the source of Paramagnetic Relaxation Enhancements (PRE) used as alternative distance constraints. Comparison of the family of structures obtained by NOE structural restraints, with that obtained by PRE and with the family of structures obtained by combining NOEs and PREs reveals that the pairwise RMSD between them are similar and comparable with the precision within each family. This work sets the stage for the structural characterization of small and dynamic paramagnetic metalloproteins opening a new paradigm in the use of NMR in structural biology

NOE-less protein structures-an alternative paradigm in Highly Paramagnetic Systems / Trindade Ines, Invernici Michele, Cantini Francesca, Louro Ricardo, Piccioli Mario. - ELETTRONICO. - (2020), pp. 0-0. [10.48550/arXiv.2002.01228]

NOE-less protein structures-an alternative paradigm in Highly Paramagnetic Systems

Invernici Michele;Cantini Francesca;Piccioli Mario
2020

Abstract

The current paradigm for protein structure determination by NMR spectroscopy has thus far been based on collecting distance restrains between pairs of nuclei in the form of nuclear Overhauser enhancements (NOE). These, have in some circumstances been supplemented with other information sources such as paramagnetic relaxation enhancements or residual dipolar couplings. Here, we report for the first time a protein structure determination by NMR without the use of NOEs. The protein PioC from Rhodopseu-domonas palustris TIE-1 is an HIPIP where the 4Fe4S cluster is paramagnetic and provides the source of Paramagnetic Relaxation Enhancements (PRE) used as alternative distance constraints. Comparison of the family of structures obtained by NOE structural restraints, with that obtained by PRE and with the family of structures obtained by combining NOEs and PREs reveals that the pairwise RMSD between them are similar and comparable with the precision within each family. This work sets the stage for the structural characterization of small and dynamic paramagnetic metalloproteins opening a new paradigm in the use of NMR in structural biology
2020
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Trindade Ines, Invernici Michele, Cantini Francesca, Louro Ricardo, Piccioli Mario
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1205006
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