The characterization of cell-mediated and humoral adaptive immune responses to SARS-CoV-2 is fundamental to understand COVID-19 progression and the development of immunological memory to the virus. In this study, we detected T cells reactive to SARS-CoV-2 proteins M, S and N, as well as serum virus-specific IgM, IgA, IgG, in nearly all SARS-CoV-2 infected individuals, but not in healthy donors. Virus-reactive T cells exhibited signs of in vivo activation, as suggested by the surface expression of immune-checkpoint molecules PD1 and TIGIT. Of note, we detected antigen-specific adaptive immune response both in asymptomatic and symptomatic SARS-CoV-2 infected subjects. More importantly, symptomatic patients displayed a significantly higher magnitude of both cell-mediated and humoral adaptive immune response to the virus, as compared to asymptomatic individuals. These findings suggest that an uncontrolled adaptive immune response contribute to the development of the life threatening inflammatory phase of the disease. Finally, this study might open the way to develop effective vaccination strategies. This article is protected by copyright. All rights reserved.

Cell-mediated and humoral adaptive immune responses to SARS-CoV-2 are lower in asymptomatic than symptomatic COVID-19 patients / Mazzoni, Alessio; Maggi, Laura; Capone, Manuela; Spinicci, Michele; Salvati, Lorenzo; Colao, Maria Grazia; Vanni, Anna; Kiros, Seble Tekle; Mencarini, Jessica; Zammarchi, Lorenzo; Mantengoli, Elisabetta; Menicacci, Lorenzo; Caldini, Eleonora; Romagnani, Sergio; Liotta, Francesco; Morettini, Alessandro; Rossolini, Gian Maria; Bartoloni, Alessandro; Cosmi, Lorenzo; Annunziato, Francesco. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - ELETTRONICO. - (2020), pp. 1-1. [10.1002/eji.202048915]

Cell-mediated and humoral adaptive immune responses to SARS-CoV-2 are lower in asymptomatic than symptomatic COVID-19 patients

Mazzoni, Alessio;Maggi, Laura;Capone, Manuela;Spinicci, Michele;Salvati, Lorenzo;Vanni, Anna;Kiros, Seble Tekle;Mencarini, Jessica;Zammarchi, Lorenzo;Mantengoli, Elisabetta;Menicacci, Lorenzo;Caldini, Eleonora;Romagnani, Sergio;Liotta, Francesco;Morettini, Alessandro;Rossolini, Gian Maria;Bartoloni, Alessandro;Cosmi, Lorenzo;Annunziato, Francesco
2020

Abstract

The characterization of cell-mediated and humoral adaptive immune responses to SARS-CoV-2 is fundamental to understand COVID-19 progression and the development of immunological memory to the virus. In this study, we detected T cells reactive to SARS-CoV-2 proteins M, S and N, as well as serum virus-specific IgM, IgA, IgG, in nearly all SARS-CoV-2 infected individuals, but not in healthy donors. Virus-reactive T cells exhibited signs of in vivo activation, as suggested by the surface expression of immune-checkpoint molecules PD1 and TIGIT. Of note, we detected antigen-specific adaptive immune response both in asymptomatic and symptomatic SARS-CoV-2 infected subjects. More importantly, symptomatic patients displayed a significantly higher magnitude of both cell-mediated and humoral adaptive immune response to the virus, as compared to asymptomatic individuals. These findings suggest that an uncontrolled adaptive immune response contribute to the development of the life threatening inflammatory phase of the disease. Finally, this study might open the way to develop effective vaccination strategies. This article is protected by copyright. All rights reserved.
2020
1
1
Goal 3: Good health and well-being for people
Mazzoni, Alessio; Maggi, Laura; Capone, Manuela; Spinicci, Michele; Salvati, Lorenzo; Colao, Maria Grazia; Vanni, Anna; Kiros, Seble Tekle; Mencarini, Jessica; Zammarchi, Lorenzo; Mantengoli, Elisabetta; Menicacci, Lorenzo; Caldini, Eleonora; Romagnani, Sergio; Liotta, Francesco; Morettini, Alessandro; Rossolini, Gian Maria; Bartoloni, Alessandro; Cosmi, Lorenzo; Annunziato, Francesco
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1213511
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