The aim of this work is to study the 3D organization of some population of interneurons within a sample of interest, previously analyzed for physiological information, using two photon fluorescence microscopy (TPFM). We exploit the high axial and radial resolution of TPFM optical sectioning (0.44 x 0.44 x 2 μm³) in combination with a protocol for tissue clearing and labeling to perform the 3D reconstruction of 300 um thick brain sections. We clear the sections with the SWITCH/TDE clearing method and label the samples with three antibodies to co-stain three different populations of inhibitory interneurons: PV (Parvalbumin), SST(Somatostatin), and VIP (Vaso Intestinal Peptide).
Molecular characterization of the interneurons in human temporal neocortex by two photon fluorescence microscopy / I. Costantini; G. Mazzamuto. - ELETTRONICO. - (2020). [10.25493/v9gw-4jg]
Molecular characterization of the interneurons in human temporal neocortex by two photon fluorescence microscopy
I. Costantini;G. Mazzamuto
2020
Abstract
The aim of this work is to study the 3D organization of some population of interneurons within a sample of interest, previously analyzed for physiological information, using two photon fluorescence microscopy (TPFM). We exploit the high axial and radial resolution of TPFM optical sectioning (0.44 x 0.44 x 2 μm³) in combination with a protocol for tissue clearing and labeling to perform the 3D reconstruction of 300 um thick brain sections. We clear the sections with the SWITCH/TDE clearing method and label the samples with three antibodies to co-stain three different populations of inhibitory interneurons: PV (Parvalbumin), SST(Somatostatin), and VIP (Vaso Intestinal Peptide).
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