The aim of this work is to reconstruct the 3D organization of neurons in a left hippocampus from sub-cellular resolution images obtained by a custom-made dual view inverted light sheet fluorescence microscope. We analyze the left hippocampus of a 99-year old woman, not affected by Alzheimer’s disease, nor hypertension, but presenting a cognitive decline, obtained from the University of Tours. The sample was cut into 72 slices of 500 um thickness before imaging. Each slice was cleared with the SWITCH/TDE method and stained with NeuN (all neurons) and GAD67 (inhibitory neurons). Imaging was performed at a resolution of 1.1 x 1.1 x 3.8 μm³.

Layer-specific excitatory and inhibitory neuronal maps of hippocampus / F. Pavone; G. Mazzamuto; I. Costantini. - ELETTRONICO. - (2020). [10.25493/1gzv-zu]

Layer-specific excitatory and inhibitory neuronal maps of hippocampus

F. Pavone;G. Mazzamuto;I. Costantini
2020

Abstract

The aim of this work is to reconstruct the 3D organization of neurons in a left hippocampus from sub-cellular resolution images obtained by a custom-made dual view inverted light sheet fluorescence microscope. We analyze the left hippocampus of a 99-year old woman, not affected by Alzheimer’s disease, nor hypertension, but presenting a cognitive decline, obtained from the University of Tours. The sample was cut into 72 slices of 500 um thickness before imaging. Each slice was cleared with the SWITCH/TDE method and stained with NeuN (all neurons) and GAD67 (inhibitory neurons). Imaging was performed at a resolution of 1.1 x 1.1 x 3.8 μm³.
Goal 3: Good health and well-being
Goal 9: Industry, Innovation, and Infrastructure
F. Pavone; G. Mazzamuto; I. Costantini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1215803
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