Layer-specific excitatory and inhibitory neuronal maps of hippocampus

The aim of this work is to reconstruct the 3D organization of neurons in a left hippocampus from sub-cellular resolution images obtained by a custom-made dual view inverted light sheet fluorescence microscope. We analyze the left hippocampus of a 99-year old woman, not affected by Alzheimer’s disease, nor hypertension, but presenting a cognitive decline, obtained from the University of Tours. The sample was cut into 72 slices of 500 um thickness before imaging. Each slice was cleared with the SWITCH/TDE method and stained with NeuN (all neurons) and GAD67 (inhibitory neurons). Imaging was performed at a resolution of 1.1 x 1.1 x 3.8 μm³.