The current pandemic emergence of novel coronavirus disease (COVID-19) poses a relevant threat to global health. SARS-CoV-2 infection is characterized by a wide range of clinical manifestations, ranging from absence of symptoms to severe forms that need intensive care treatment. Here, plasma-EDTA samples of 30 patients compared with age- and sex-matched controls were analyzed via untargeted nuclear magnetic resonance (NMR)-based metabolomics and lipidomics. With the same approach, the effect of tocilizumab administration was evaluated in a subset of patients. Despite the heterogeneity of the clinical symptoms, COVID-19 patients are characterized by common plasma metabolomic and lipidomic signatures (91.7% and 87.5% accuracy, respectively, when compared to controls). Tocilizumab treatment resulted in at least partial reversion of the metabolic alterations due to SARS-CoV-2 infection. In conclusion, NMR-based metabolomic and lipidomic profiling provides novel insights into the pathophysiological mechanism of human response to SARS-CoV-2 infection and to monitor treatment outcomes.

Metabolomic/lipidomic profiling of COVID-19 and individual response to tocilizumab / Meoni, Gaia; Ghini, Veronica; Maggi, Laura; Vignoli, Alessia; Mazzoni, Alessio; Salvati, Lorenzo; Capone, Manuela; Vanni, Anna; Tenori, Leonardo; Fontanari, Paolo; Lavorini, Federico; Peris, Adriano; Bartoloni, Alessandro; Liotta, Francesco; Cosmi, Lorenzo; Luchinat, Claudio; Annunziato, Francesco; Turano, Paola. - In: PLOS PATHOGENS. - ISSN 1553-7374. - ELETTRONICO. - 17:(2021), pp. e1009243-e1009243. [10.1371/journal.ppat.1009243]

Metabolomic/lipidomic profiling of COVID-19 and individual response to tocilizumab

Meoni, Gaia;Ghini, Veronica;Maggi, Laura;Vignoli, Alessia;Mazzoni, Alessio;Salvati, Lorenzo;Capone, Manuela;Vanni, Anna;Tenori, Leonardo;Fontanari, Paolo;Lavorini, Federico;Peris, Adriano;Bartoloni, Alessandro;Liotta, Francesco;Cosmi, Lorenzo;Luchinat, Claudio;Annunziato, Francesco;Turano, Paola
2021

Abstract

The current pandemic emergence of novel coronavirus disease (COVID-19) poses a relevant threat to global health. SARS-CoV-2 infection is characterized by a wide range of clinical manifestations, ranging from absence of symptoms to severe forms that need intensive care treatment. Here, plasma-EDTA samples of 30 patients compared with age- and sex-matched controls were analyzed via untargeted nuclear magnetic resonance (NMR)-based metabolomics and lipidomics. With the same approach, the effect of tocilizumab administration was evaluated in a subset of patients. Despite the heterogeneity of the clinical symptoms, COVID-19 patients are characterized by common plasma metabolomic and lipidomic signatures (91.7% and 87.5% accuracy, respectively, when compared to controls). Tocilizumab treatment resulted in at least partial reversion of the metabolic alterations due to SARS-CoV-2 infection. In conclusion, NMR-based metabolomic and lipidomic profiling provides novel insights into the pathophysiological mechanism of human response to SARS-CoV-2 infection and to monitor treatment outcomes.
2021
17
e1009243
e1009243
Meoni, Gaia; Ghini, Veronica; Maggi, Laura; Vignoli, Alessia; Mazzoni, Alessio; Salvati, Lorenzo; Capone, Manuela; Vanni, Anna; Tenori, Leonardo; Fontanari, Paolo; Lavorini, Federico; Peris, Adriano; Bartoloni, Alessandro; Liotta, Francesco; Cosmi, Lorenzo; Luchinat, Claudio; Annunziato, Francesco; Turano, Paola
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1225146
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