Leaf epidermal cells make ideal specimens for the investigation of the plant secretory pathway in that it is relatively easy to tag with fluorescent proteins and visualize in vivo the various organelles of the pathway. A number of techniques can be employed to identify and study proteins within the endomembrane organelles and to study their dynamics and interactions. Here, we discuss the most commonly used approaches to express proteins within arabidopsis and tobacco leaves, the use of mutant screens to identify trafficking proteins, and the use of two in vivo techniques, Fluorescence recovery after photobleaching and Förster resonance energy transfer, to study protein dynamics in plant cells. © 2013 Elsevier Inc.
Endomembrane and golgi traffic in plant cells / Stefano G.; Osterrieder A.; Hawes C.; Brandizzi F.. - In: METHODS IN CELL BIOLOGY. - ISSN 0091-679X. - ELETTRONICO. - 118:(2013), pp. 69-83. [10.1016/B978-0-12-417164-0.00005-7]
Endomembrane and golgi traffic in plant cells
Stefano G.Membro del Collaboration Group
;
2013
Abstract
Leaf epidermal cells make ideal specimens for the investigation of the plant secretory pathway in that it is relatively easy to tag with fluorescent proteins and visualize in vivo the various organelles of the pathway. A number of techniques can be employed to identify and study proteins within the endomembrane organelles and to study their dynamics and interactions. Here, we discuss the most commonly used approaches to express proteins within arabidopsis and tobacco leaves, the use of mutant screens to identify trafficking proteins, and the use of two in vivo techniques, Fluorescence recovery after photobleaching and Förster resonance energy transfer, to study protein dynamics in plant cells. © 2013 Elsevier Inc.
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