Purpose Our purpose was to better understand the pathogenesis of seizures associated with angiocentric glioma. Angiocentric glioma is an indolent and rare low-grade glioma. Its typical clinical presentation is with epileptic seizures. The pathogenesis of tumor-associated seizures is poorly understood. Among the possible pathomechanisms, the increased neurotoxic concentrations of the glutamate has been proposed. Glutamate transporters, pyruvate carboxylase and glutamine synthetase are involved in maintaining the physiological concentration of glutamate in the inter synaptic spaces. Methods We evaluated the immunohistochemical expression of EAAT2 (the most important glutamate transporter), pyruvate carboxylase and glutamine synthetase in 17 angiocentric gliomas. Results EAAT2 was never expressed (0%) in the neoplastic cells in none of the cases studied. Pyruvate carboxylase was expressed in the cytoplasm of the neoplastic cells in 16/17 cases (94%). Glutamine synthetase was expressed in the cytoplasm of the neoplastic cells in 15/17 cases (88%). Conclusion The net result of this enzymatic expression, in particular considering the loss of EAAT2, could be an increased glutamate concentration in the synaptic clef, which might increase local network excitability initially involving intratumoral neurons. The observation that the angiocentric glioma-associated epilepsy might be at least in part related to EAAT2 deficiency opens up interesting therapeutic perspectives.

Angiocentric glioma-associated seizures: The possible role of EATT2, pyruvate carboxylase and glutamine synthetase / Anna Maria Buccoliero, Chiara Caporalini, Mirko Scagnet, Federico Mussa, Flavio Giordano, Iacopo Sardi, Irene Migliastro, Selene Moscardi, Valerio Conti, Carmen Barba, Manila Antonelli, Francesca Gianno, Sabrina Rossi, Francesca Diomedi-Camassei, Marco Gessi, Vittoria Donofrio, Luca Bertero, Felice Giangaspero, Mariarita Santi, Eleonora Aronica, Lorenzo Genitori, Renzo Guerrini. - In: SEIZURE. - ISSN 1059-1311. - ELETTRONICO. - 16:(2021), pp. 152-154. [10.1016/j.seizure.2021.02.014]

Angiocentric glioma-associated seizures: The possible role of EATT2, pyruvate carboxylase and glutamine synthetase

Flavio Giordano
Writing – Review & Editing
;
Carmen Barba
Writing – Review & Editing
;
Renzo Guerrini
Writing – Review & Editing
2021

Abstract

Purpose Our purpose was to better understand the pathogenesis of seizures associated with angiocentric glioma. Angiocentric glioma is an indolent and rare low-grade glioma. Its typical clinical presentation is with epileptic seizures. The pathogenesis of tumor-associated seizures is poorly understood. Among the possible pathomechanisms, the increased neurotoxic concentrations of the glutamate has been proposed. Glutamate transporters, pyruvate carboxylase and glutamine synthetase are involved in maintaining the physiological concentration of glutamate in the inter synaptic spaces. Methods We evaluated the immunohistochemical expression of EAAT2 (the most important glutamate transporter), pyruvate carboxylase and glutamine synthetase in 17 angiocentric gliomas. Results EAAT2 was never expressed (0%) in the neoplastic cells in none of the cases studied. Pyruvate carboxylase was expressed in the cytoplasm of the neoplastic cells in 16/17 cases (94%). Glutamine synthetase was expressed in the cytoplasm of the neoplastic cells in 15/17 cases (88%). Conclusion The net result of this enzymatic expression, in particular considering the loss of EAAT2, could be an increased glutamate concentration in the synaptic clef, which might increase local network excitability initially involving intratumoral neurons. The observation that the angiocentric glioma-associated epilepsy might be at least in part related to EAAT2 deficiency opens up interesting therapeutic perspectives.
2021
16
152
154
Anna Maria Buccoliero, Chiara Caporalini, Mirko Scagnet, Federico Mussa, Flavio Giordano, Iacopo Sardi, Irene Migliastro, Selene Moscardi, Valerio Con...espandi
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S1059131121000480-mainext.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 549.35 kB
Formato Adobe PDF
549.35 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1226174
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 11
social impact