Although neuronal density analysis on human brain slices is available from stereological studies, data on the spatial distribution of neurons in 3D are still missing. Since the neuronal organization is very inhomogeneous in the cerebral cortex, it is critical to map all neurons in a given volume rather than relying on sparse sampling methods. To achieve this goal, we implement a new tissue transformation protocol to clear and label human brain tissues and we exploit the high-resolution optical sectioning of two-photon fluorescence microscopy to perform 3D mesoscopic reconstruction. We perform neuronal mapping of 100mm3 human brain samples and evaluate the volume and density distribution of neurons from various areas of the cortex originating from different subjects (young, adult, and elderly, both healthy and pathological). The quantitative evaluation of the density in combination with the mean volume of the thousands of neurons identified within the specimens, allow us to determine the layer-specific organization of the cerebral architecture.

Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture / Irene Costantini and Giacomo Mazzamuto and Matteo Roffilli and Annunziatina Laurino and Filippo Maria Castelli and Mattia Neri and Giovanni Lughi and Andrea Simonetto and Erica Lazzeri and Luca Pesce and Christophe Destrieux and Ludovico Silvestri and Valerio Conti and Renzo Guerrini and Francesco Saverio Pavone. - In: BIOMEDICAL OPTICS EXPRESS. - ISSN 2156-7085. - ELETTRONICO. - 12:(2021), pp. 3684-3699. [10.1364/BOE.415555]

Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture

Irene Costantini
;
Giacomo Mazzamuto;Annunziatina Laurino;Filippo Maria Castelli;Luca Pesce;Ludovico Silvestri;Valerio Conti;Renzo Guerrini;Francesco Saverio Pavone
2021

Abstract

Although neuronal density analysis on human brain slices is available from stereological studies, data on the spatial distribution of neurons in 3D are still missing. Since the neuronal organization is very inhomogeneous in the cerebral cortex, it is critical to map all neurons in a given volume rather than relying on sparse sampling methods. To achieve this goal, we implement a new tissue transformation protocol to clear and label human brain tissues and we exploit the high-resolution optical sectioning of two-photon fluorescence microscopy to perform 3D mesoscopic reconstruction. We perform neuronal mapping of 100mm3 human brain samples and evaluate the volume and density distribution of neurons from various areas of the cortex originating from different subjects (young, adult, and elderly, both healthy and pathological). The quantitative evaluation of the density in combination with the mean volume of the thousands of neurons identified within the specimens, allow us to determine the layer-specific organization of the cerebral architecture.
2021
12
3684
3699
Irene Costantini and Giacomo Mazzamuto and Matteo Roffilli and Annunziatina Laurino and Filippo Maria Castelli and Mattia Neri and Giovanni Lughi and Andrea Simonetto and Erica Lazzeri and Luca Pesce and Christophe Destrieux and Ludovico Silvestri and Valerio Conti and Renzo Guerrini and Francesco Saverio Pavone
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1238155
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