Background: Cytokines and chemokines, secreted in the intrauterine environment, are fundamental for the molecular crosstalk between endometrium and preimplantation embryo. Whether Vitamin D can have a beneficial effect on endometrial district and immune cells is still unclear. To fill this gap, the aim of this study was to explore if Vitamin D supplementation can act on intrauterine milieu as immuno-modulator. Methods: We present a report of a secondary outcome from the SUNDRO clinical trial, a multi-center randomized double-blinded trial aimed to explore the effects of Vitamin D replacement (a single dose of 600,000 IU of 25-hydroxy Vitamin D or placebo) in insufficient women undergoing autologous ART cycles. Inclusion criteria were female age between 18-39 years, with body mass index between 18 and 25 Kg/m2. Uterine fluid samples were collected during the secretory phase of the menstrual cycle that proceeded the oocyte retrieval. The quantitative determination of twenty-seven cytokines in endometrial secretion samples was performed by using a multiplex immunoassay. Results: Forty-nine uterine fluid samples (UF) were collected during the secretory phase of the menstrual cycle prior to oocyte retrieval. Our data revealed no differences in UF composition of Vitamin D supplemented women (n=17) compared with the placebo group (n=32), also when protein normalization was performed. In addition, no significant differences were found in mediators relative content of UFs from women who conceived (n=19) compared with the non-pregnant group (n=30). Conclusions: The explored in vivo vitamin D replacement regimen is unlikely to directly influence the cytokine and chemokine endometrial milieu.

Oral Vitamin D Supplementation does not Impact Cytokine Levels in Uterine Fluid of Women Undergoing Assisted Reproduction Technology (ART) / Greta Chiara Cermisoni, Marco Reschini, Marie-Pierre Piccinni, Letizia Lombardelli, Federica Logiodice, Veronica Sarais, Elisa Giacomini, Simona Signorelli, Edgardo Somigliana, Paola Viganò. - In: BMC PREGNANCY AND CHILDBIRTH. - ISSN 1471-2393. - ELETTRONICO. - (2021), pp. 1-24. [10.21203/rs.3.rs-271816/v1]

Oral Vitamin D Supplementation does not Impact Cytokine Levels in Uterine Fluid of Women Undergoing Assisted Reproduction Technology (ART)

Marie-Pierre Piccinni;Letizia Lombardelli;Federica Logiodice;
2021

Abstract

Background: Cytokines and chemokines, secreted in the intrauterine environment, are fundamental for the molecular crosstalk between endometrium and preimplantation embryo. Whether Vitamin D can have a beneficial effect on endometrial district and immune cells is still unclear. To fill this gap, the aim of this study was to explore if Vitamin D supplementation can act on intrauterine milieu as immuno-modulator. Methods: We present a report of a secondary outcome from the SUNDRO clinical trial, a multi-center randomized double-blinded trial aimed to explore the effects of Vitamin D replacement (a single dose of 600,000 IU of 25-hydroxy Vitamin D or placebo) in insufficient women undergoing autologous ART cycles. Inclusion criteria were female age between 18-39 years, with body mass index between 18 and 25 Kg/m2. Uterine fluid samples were collected during the secretory phase of the menstrual cycle that proceeded the oocyte retrieval. The quantitative determination of twenty-seven cytokines in endometrial secretion samples was performed by using a multiplex immunoassay. Results: Forty-nine uterine fluid samples (UF) were collected during the secretory phase of the menstrual cycle prior to oocyte retrieval. Our data revealed no differences in UF composition of Vitamin D supplemented women (n=17) compared with the placebo group (n=32), also when protein normalization was performed. In addition, no significant differences were found in mediators relative content of UFs from women who conceived (n=19) compared with the non-pregnant group (n=30). Conclusions: The explored in vivo vitamin D replacement regimen is unlikely to directly influence the cytokine and chemokine endometrial milieu.
2021
1
24
Goal 3: Good health and well-being for people
Greta Chiara Cermisoni, Marco Reschini, Marie-Pierre Piccinni, Letizia Lombardelli, Federica Logiodice, Veronica Sarais, Elisa Giacomini, Simona Signo...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1240307
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