Ultrasound (US) stimulation of neural activity has gained increasing attention in the scientific community, especially thanks to its advantages, first and foremost non-invasiveness. Little is known, however, about the relationship between the properties of the target neuronal populations and their responsiveness to this form of stimulation in vivo. Here, we report cortex-wide US-induced activity patterns of zebrafish larvae recorded through calcium imaging microscopy. We were able to induce a reliable neuronal activation monotonically increasing with applied pressure. As expected by previous results, activation probability grows with stimulus duration. Moreover, a spatial analysis of the responsiveness showed that it was highly area-specific, indicating a role of neuronal type and architecture in US sensitivity. Control experiments finally proved that sensory perception of US-induced water movements did not account for the whole neural activation, highlighting the role of direct neuronal stimulation. These results provide a frame for US studies on zebrafishes, characterizing the relationship between stimulation parameters and local responsiveness. Future studies will focus on the link between area-specific neuronal properties and US sensitivity to shed light on the biophysics of US neuromodulation.
Ultrasonic neural stimulation of zebrafish larvae reveals region-specific calcium imaging activation patterns / Meneghetti, N.; Dedola, F.; Gavryusev, V.; Sancataldo, G.; Turrini, L.; de Vito, G.; Vanzi, F.; Carpaneto, J.; Cutrone, A.; Saverio Pavone, F.; Micera, S.; Mazzoni, A.. - ELETTRONICO. - (2021), pp. 0-0. (Intervento presentato al convegno GNB 2020 - Seventh National Congress of Bioengineering tenutosi a Trieste nel 9-11 Giugno 2021).
Ultrasonic neural stimulation of zebrafish larvae reveals region-specific calcium imaging activation patterns
Gavryusev, V.;Sancataldo, G.;Turrini, L.;de Vito, G.;Vanzi, F.;Saverio Pavone, F.;
2021
Abstract
Ultrasound (US) stimulation of neural activity has gained increasing attention in the scientific community, especially thanks to its advantages, first and foremost non-invasiveness. Little is known, however, about the relationship between the properties of the target neuronal populations and their responsiveness to this form of stimulation in vivo. Here, we report cortex-wide US-induced activity patterns of zebrafish larvae recorded through calcium imaging microscopy. We were able to induce a reliable neuronal activation monotonically increasing with applied pressure. As expected by previous results, activation probability grows with stimulus duration. Moreover, a spatial analysis of the responsiveness showed that it was highly area-specific, indicating a role of neuronal type and architecture in US sensitivity. Control experiments finally proved that sensory perception of US-induced water movements did not account for the whole neural activation, highlighting the role of direct neuronal stimulation. These results provide a frame for US studies on zebrafishes, characterizing the relationship between stimulation parameters and local responsiveness. Future studies will focus on the link between area-specific neuronal properties and US sensitivity to shed light on the biophysics of US neuromodulation.File | Dimensione | Formato | |
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