Detection of canine and equine procalcitonin for sepsis diagnosis in veterinary clinic by the development of novel MIP-based SPR biosensors

Talanta 230 (2021) 122347 Available online 23 March 2021 0039-9140/© 2021 Elsevier B.V. All rights reserved.Detection of canine and equine procalcitonin for sepsis diagnosis in veterinary clinic by the development of novel MIP-based SPR biosensors F. Battaglia b, V. Baldoneschi a, V. Meucci b,*, L. Intorre b, M. Minunni a, S. Scarano a,** a Department of Chemistry “Ugo Schiff”, University of Florence, 50019, Sesto Fiorentino, (FI), Italy b Department of Veterinary Science, University of Pisa, 56122, Via Livornese, (PI), Italy A R T I C L E I N F O Keywords: A B S T R A C T Procalcitonin (PCT) has emerged as a promising biomarker for the rapid identification of sepsis both in human and veterinary medicine. Nevertheless, the only analytical method currently available for the detection of PCT in veterinary species, is represented by immunoassays, useful only for research purposes. In this work, we report the development of two biosensors which utilize molecularly imprinted polymers (MIPs) for the detection of canine and equine PCT. Dopamine (DA) and norepinephrine (NE) were used as monomers for the synthesis of the MIP films on surface plasmon resonance (SPR) gold chips and the imprinting efficiency of canine and equine PCT in terms of binding affinity toward the analyte, selectivity, and sensitivity were compared. After optimization in buffer conditions, PCTs calibration was successfully achieved also in animal plasma, with good specificity and reproducibility. More effective protein binding and imprinting was obtained with polynorepinephrine (PNE) for both PCTs, and the SPR biosensors were able to detect the biomarkers in plasma with a LOD of 15 ng mL 1 and 30 ng mL 1 respectively for equine and canine PCT