An innovative two-photon microscope exploiting a line-shaped illumination has been recently devised and then implemented. Such configuration allows to carry out a real-time detection by means of standard CCD cameras and is able to maintain the same resolution as commonly used point-scanning devices, thus overcoming what is usually regarded as the main limitation of line-scanning microscopes. Here, we provide an overview of the applications in which this device has been tested and has proved to be a flexible and efficient tool, namely imaging of biological samples, in-depth sample reconstruction, two-photon spectra detection, and dye cross-section measurements. These results demonstrate that the considered setup is promising for future developments in many areas of research and applications.

Line-Shaped Illumination: A Promising Configuration for a Flexible Two-Photon Microscopy Setup / Jacopo Parravicini, Elton Hasani, Luca Tartara. - In: APPLIED SCIENCES. - ISSN 2076-3417. - ELETTRONICO. - 12:(2022), pp. 3938-3938. [10.3390/app 12083938]

Line-Shaped Illumination: A Promising Configuration for a Flexible Two-Photon Microscopy Setup

Jacopo Parravicini
;
2022

Abstract

An innovative two-photon microscope exploiting a line-shaped illumination has been recently devised and then implemented. Such configuration allows to carry out a real-time detection by means of standard CCD cameras and is able to maintain the same resolution as commonly used point-scanning devices, thus overcoming what is usually regarded as the main limitation of line-scanning microscopes. Here, we provide an overview of the applications in which this device has been tested and has proved to be a flexible and efficient tool, namely imaging of biological samples, in-depth sample reconstruction, two-photon spectra detection, and dye cross-section measurements. These results demonstrate that the considered setup is promising for future developments in many areas of research and applications.
2022
12
3938
3938
Jacopo Parravicini, Elton Hasani, Luca Tartara
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1264415
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