Circulating tumor cells detection and ARV7 expression are associated with worse clinical outcomes in metastatic Castration- Resistant Prostate Cancer (mCRPC) undergoing Androgen Receptor Targeted Agents. ARFL, PSMA and PSA may help to refine prognostic models. In our institution, a prospective observational trial testing CTC detection in mCPRC undergo- ing I line ARTA therapy terminated the planned enrollment in 2020. Here, we present a pre-planned interim analysis with 18 months of median follow-up. RT-qPCR was used to determine the CTC expression of PSA, PSMA, AR and ARV7 before starting ARTA. PSA-drop, Progression-Free and Overall Survival (PFS and OS) and their correlation with CTC detection were reported. Forty-four patients were included. CTC were detected in 43.2% of patients, of whom 8.94% expressed PSA, 15.78% showed ARV7, 63.15% and 73.68% displayed ARFL and PSMA, respectively. Biochemical response was significantly improved in CTC + vs CTC− patients, with median PSA-drop of 18.5 vs 2.5 ng/ml (p = 0.03). After a median follow-up of 18 months, 50% of patients progressed. PFS was significantly longer in CTC- patients (NR vs 16 months). Eight (18.2%) patients died, a non-significant trend in terms of OS was detected in favor of CTC− patients (NR vs 29 months, p = 0.05). AR, PSA and PSMA expression in CTC + had no significant impact on PSA-drop, PFS or OS. PRIMERA-trial confirmed the CTC detection predictive importance in mCRPC patients.

Prospective assessment of AR splice variant and multi-biomarker expression on circulating tumor cells of mCRPC patients undergoing androgen receptor targeted agents: interim analysis of PRIMERA trial (NCT04188275) / Giulio Francolini, Mauro Loi, Lucia Pia Ciccone, Beatrice Detti, Vanessa Di Cataldo, Pamela Pinzani, Francesca Salvianti, Giulia Salvatore, Mariangela Sottili, Costanza Santini, Giulio Frosini, Luca Visani, Luca Burchini, Chiara Mattioli, Andrea Gaetano Allegra, Marianna Valzano, Cecilia Cerbai, Michele Aquilano, Viola Salvestrini, Isacco Desideri, Monica Mangoni, Icro Meattini, Lorenzo Livi. - In: MEDICAL ONCOLOGY. - ISSN 1357-0560. - ELETTRONICO. - 39:(2022), pp. 119-127. [10.1007/s12032-022-01756-2]

Prospective assessment of AR splice variant and multi-biomarker expression on circulating tumor cells of mCRPC patients undergoing androgen receptor targeted agents: interim analysis of PRIMERA trial (NCT04188275)

Giulio Francolini
;
Mauro Loi;Lucia Pia Ciccone;Beatrice Detti;Vanessa Di Cataldo;Pamela Pinzani;Francesca Salvianti;Giulia Salvatore;Mariangela Sottili;Costanza Santini;Giulio Frosini;Luca Visani;Luca Burchini;Chiara Mattioli;Marianna Valzano;Cecilia Cerbai;Michele Aquilano;Viola Salvestrini;Isacco Desideri;Monica Mangoni;Icro Meattini;Lorenzo Livi
2022

Abstract

Circulating tumor cells detection and ARV7 expression are associated with worse clinical outcomes in metastatic Castration- Resistant Prostate Cancer (mCRPC) undergoing Androgen Receptor Targeted Agents. ARFL, PSMA and PSA may help to refine prognostic models. In our institution, a prospective observational trial testing CTC detection in mCPRC undergo- ing I line ARTA therapy terminated the planned enrollment in 2020. Here, we present a pre-planned interim analysis with 18 months of median follow-up. RT-qPCR was used to determine the CTC expression of PSA, PSMA, AR and ARV7 before starting ARTA. PSA-drop, Progression-Free and Overall Survival (PFS and OS) and their correlation with CTC detection were reported. Forty-four patients were included. CTC were detected in 43.2% of patients, of whom 8.94% expressed PSA, 15.78% showed ARV7, 63.15% and 73.68% displayed ARFL and PSMA, respectively. Biochemical response was significantly improved in CTC + vs CTC− patients, with median PSA-drop of 18.5 vs 2.5 ng/ml (p = 0.03). After a median follow-up of 18 months, 50% of patients progressed. PFS was significantly longer in CTC- patients (NR vs 16 months). Eight (18.2%) patients died, a non-significant trend in terms of OS was detected in favor of CTC− patients (NR vs 29 months, p = 0.05). AR, PSA and PSMA expression in CTC + had no significant impact on PSA-drop, PFS or OS. PRIMERA-trial confirmed the CTC detection predictive importance in mCRPC patients.
2022
39
119
127
Giulio Francolini, Mauro Loi, Lucia Pia Ciccone, Beatrice Detti, Vanessa Di Cataldo, Pamela Pinzani, Francesca Salvianti, Giulia Salvatore, Mariange...espandi
File in questo prodotto:
File Dimensione Formato  
Francolini2022_Article_ProspectiveAssessmentOfARSplic.pdf

accesso aperto

Tipologia: Pdf editoriale (Version of record)
Licenza: Creative commons
Dimensione 741.49 kB
Formato Adobe PDF
741.49 kB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1277405
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact