Recurrent infection by Clostridioides difficile has recently been treated by fecal microbiota transplantation (FMT). As viable SARS-CoV-2 was recovered from stool of asymptomatic individuals, the FMT procedure could be a potential risk of SARS-CoV-2 transmission, thus underlying the need to reliably detect SARS-CoV-2 in stool. Here, we performed a multicentric study to explore performances of two commercially available assays for detection of SARS-CoV-2 RNA in stool of potential FMT donors. In three hospitals, 180 stool samples were spiked with serial 10-fold dilutions of a SARS-CoV-2 inactivated lysate to evaluate the Seegene Allplex (TM) SARS-CoV-2 (SC2) and SARS-CoV-2/FluA/FluB/RSV (SC2FABR) Assays for the detection of viral RNA in stool of FMT donors. The results revealed that both assays detected down to 2 TCID50/mL with comparable limit of detection values, SC2 showing more consistent target positivity rate than SC2FABR. Beyond high amplification efficiency, correlation between C-T values and log concentrations of inactivated viral lysates showed R-2 values ranging from 0.88 to 0.90 and from 0.87 to 0.91 for the SC2 and SC2FABR assay, respectively. The present results demonstrate that both methods are highly reproducible, sensitive, and accurate for SARS-CoV-2 RNA detection in stool, suggesting a potential use in FMT-donor screening.

Validation of Two Commercial Multiplex Real-Time PCR Assays for Detection of SARS-CoV-2 in Stool Donors for Fecal Microbiota Transplantation / Di Pilato, Vincenzo; Morecchiato, Fabio; Rizzato, Cosmeri; Quaranta, Gianluca; Fais, Roberta; Gandolfo, Claudia; Antonelli, Alberto; Cusi, Maria Grazia; Pistello, Mauro; Rossolini, Gian Maria; Sanguinetti, Maurizio; Lupetti, Antonella; Masucci, Luca. - In: MICROORGANISMS. - ISSN 2076-2607. - ELETTRONICO. - 10:(2022), pp. 284-284. [10.3390/microorganisms10020284]

Validation of Two Commercial Multiplex Real-Time PCR Assays for Detection of SARS-CoV-2 in Stool Donors for Fecal Microbiota Transplantation

Di Pilato, Vincenzo;Morecchiato, Fabio;Antonelli, Alberto;Rossolini, Gian Maria;
2022

Abstract

Recurrent infection by Clostridioides difficile has recently been treated by fecal microbiota transplantation (FMT). As viable SARS-CoV-2 was recovered from stool of asymptomatic individuals, the FMT procedure could be a potential risk of SARS-CoV-2 transmission, thus underlying the need to reliably detect SARS-CoV-2 in stool. Here, we performed a multicentric study to explore performances of two commercially available assays for detection of SARS-CoV-2 RNA in stool of potential FMT donors. In three hospitals, 180 stool samples were spiked with serial 10-fold dilutions of a SARS-CoV-2 inactivated lysate to evaluate the Seegene Allplex (TM) SARS-CoV-2 (SC2) and SARS-CoV-2/FluA/FluB/RSV (SC2FABR) Assays for the detection of viral RNA in stool of FMT donors. The results revealed that both assays detected down to 2 TCID50/mL with comparable limit of detection values, SC2 showing more consistent target positivity rate than SC2FABR. Beyond high amplification efficiency, correlation between C-T values and log concentrations of inactivated viral lysates showed R-2 values ranging from 0.88 to 0.90 and from 0.87 to 0.91 for the SC2 and SC2FABR assay, respectively. The present results demonstrate that both methods are highly reproducible, sensitive, and accurate for SARS-CoV-2 RNA detection in stool, suggesting a potential use in FMT-donor screening.
10
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Di Pilato, Vincenzo; Morecchiato, Fabio; Rizzato, Cosmeri; Quaranta, Gianluca; Fais, Roberta; Gandolfo, Claudia; Antonelli, Alberto; Cusi, Maria Grazia; Pistello, Mauro; Rossolini, Gian Maria; Sanguinetti, Maurizio; Lupetti, Antonella; Masucci, Luca
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1285846
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