The investigation of aqueous solutions containing biomolecules as a function of thermodynamic parameters, such as the pressure, is crucial for understanding biological processes. Here we report the first low frequency spectra of 1.5 M aqueous glycine from ambient pressure up to 8 kbar, i.e. in the pressure range which is crucial for understanding biological processes under extreme conditions. We observe a linear pressure dependent blue shift of the specific N-C-C-O open/close mode at ∼320 cm-1 indicating an increasing compression of the solvated glycine. In contrast, the characteristic peak of the hydrogen bond hydration water network centered, at ambient conditions, at ∼184 cm-1 non-linearly blue shifts with increasing pressure, as well, but with a slower rate than the intramolecular peak. This indicates that the macroscopic liquid-solid phase transition observed above 8 kbar pressure is driven by hydrated glycine as solidification nucleus.
Does hydrated glycine act as solidification nucleus at multi-kilobar conditions? / Alfarano S.R.; Vondracek H.; Sebastiani F.; Novelli F.; Hoberg C.; Kolling I.; Brubach J.-B.; Roy P.; Schwaab G.; Havenith M.. - In: BIOPHYSICAL CHEMISTRY. - ISSN 0301-4622. - STAMPA. - 253:(2019), pp. 106215.1-106215.5. [10.1016/j.bpc.2019.106215]
Does hydrated glycine act as solidification nucleus at multi-kilobar conditions?
Sebastiani F.;
2019
Abstract
The investigation of aqueous solutions containing biomolecules as a function of thermodynamic parameters, such as the pressure, is crucial for understanding biological processes. Here we report the first low frequency spectra of 1.5 M aqueous glycine from ambient pressure up to 8 kbar, i.e. in the pressure range which is crucial for understanding biological processes under extreme conditions. We observe a linear pressure dependent blue shift of the specific N-C-C-O open/close mode at ∼320 cm-1 indicating an increasing compression of the solvated glycine. In contrast, the characteristic peak of the hydrogen bond hydration water network centered, at ambient conditions, at ∼184 cm-1 non-linearly blue shifts with increasing pressure, as well, but with a slower rate than the intramolecular peak. This indicates that the macroscopic liquid-solid phase transition observed above 8 kbar pressure is driven by hydrated glycine as solidification nucleus.File | Dimensione | Formato | |
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