This article presents surface enhanced Raman spectra of an important model protein, namely, wheat germ agglutinin. To obtain these spectra, a novel substrate for the protein has been developed. The substrate is based on gold nanospheroids, produced by wet synthesis. The nanoparticles are then functionalized by reaction with a solution of β-D-thioglucose to increase the affinity of the agglutinin for the surface. When nanoparticle films are placed in contact with diluted agglutinin solutions, vibrational bands of the protein appear and can be easily discriminated from those of the substrate. The Raman bands of the substrate and of the protein have then been assigned correlatively. On this basis, we conclude that the interaction between the agglutinin and the nanoparticles is selectively detected only when the metal surface is suitably functionalized.

Thioglucose functionalized gold nanoparticles as active substrates for surface enhanced Raman spectroscopy of lectins / Gellini, Cristina; Feis, Alessandro. - In: VIBRATIONAL SPECTROSCOPY. - ISSN 0924-2031. - ELETTRONICO. - 123:(2022), pp. 103468.0-103468.0. [10.1016/j.vibspec.2022.103468]

Thioglucose functionalized gold nanoparticles as active substrates for surface enhanced Raman spectroscopy of lectins

Gellini, Cristina;Feis, Alessandro
2022

Abstract

This article presents surface enhanced Raman spectra of an important model protein, namely, wheat germ agglutinin. To obtain these spectra, a novel substrate for the protein has been developed. The substrate is based on gold nanospheroids, produced by wet synthesis. The nanoparticles are then functionalized by reaction with a solution of β-D-thioglucose to increase the affinity of the agglutinin for the surface. When nanoparticle films are placed in contact with diluted agglutinin solutions, vibrational bands of the protein appear and can be easily discriminated from those of the substrate. The Raman bands of the substrate and of the protein have then been assigned correlatively. On this basis, we conclude that the interaction between the agglutinin and the nanoparticles is selectively detected only when the metal surface is suitably functionalized.
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Gellini, Cristina; Feis, Alessandro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1290291
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