Lactate is used as a biomarker of the tissue oxygenation level for the evaluation of different pathologies or for the assessment of physical performances. The present work describes the application of a previously developed platform for the colorimetric enzymatic detection of l-lactate using a smartphone-based technology. The sensor is based on the lactate oxidase-horseradish peroxidase (LOx-HRP) enzymes immobilized by adsorption on a poly (aniline-co-anthranilic acid) (p(ANI-co-AA)) composite polymer. The unique colorimetric properties of this film were exploited for the detection of lactate using a reversible redox reaction catalyzed by the adsorbed enzymes, which generates a color change of the polymer from green to blue only when the substrate is present. The sensor response, consisting in changes of the polymer optical features, was easily evaluated using the free-of-charge Color Grab application for Android OS installed on a smartphone. The developed sensor was optimized in regard to several experimental parameters and the color variation of the polymer was also monitored by spectrophotometric measurements. Finally, the developed biosensor was employed for the detection and quantification of l-lactate in different media relevant for clinical practice.

Enzymatic Detection of l-Lactate with a Smart Colorimetric Sensor / Selvolini G.; Dragan A.-M.; Melinte G.; Cristea C.; Marrazza G.. - ELETTRONICO. - 918 LNEE:(2023), pp. 3-7. ((Intervento presentato al convegno Annual Italian Conference on Sensors and Microsystems, AISEM 2021 [10.1007/978-3-031-08136-1_1].

Enzymatic Detection of l-Lactate with a Smart Colorimetric Sensor

Selvolini G.;Melinte G.;Marrazza G.
2023

Abstract

Lactate is used as a biomarker of the tissue oxygenation level for the evaluation of different pathologies or for the assessment of physical performances. The present work describes the application of a previously developed platform for the colorimetric enzymatic detection of l-lactate using a smartphone-based technology. The sensor is based on the lactate oxidase-horseradish peroxidase (LOx-HRP) enzymes immobilized by adsorption on a poly (aniline-co-anthranilic acid) (p(ANI-co-AA)) composite polymer. The unique colorimetric properties of this film were exploited for the detection of lactate using a reversible redox reaction catalyzed by the adsorbed enzymes, which generates a color change of the polymer from green to blue only when the substrate is present. The sensor response, consisting in changes of the polymer optical features, was easily evaluated using the free-of-charge Color Grab application for Android OS installed on a smartphone. The developed sensor was optimized in regard to several experimental parameters and the color variation of the polymer was also monitored by spectrophotometric measurements. Finally, the developed biosensor was employed for the detection and quantification of l-lactate in different media relevant for clinical practice.
Annual Italian Conference on Sensors and Microsystems, AISEM 2021
Annual Italian Conference on Sensors and Microsystems, AISEM 2021
Selvolini G.; Dragan A.-M.; Melinte G.; Cristea C.; Marrazza G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1290929
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