The main reasons for performing derivatization in capillary electrophoresis are largely the same as for liquid chromatography, however there are specific aspects in electrokinetic separations where derivatization plays specific roles. The review is focused on the articles published in the past 5 years with the aim to highlight this unicity. Derivatization is mainly applied to improve the inherent low sensitivity of capillary electrophoresis when optical detection is used and the introduction of originally developed derivatization approaches have been addressed mainly to the detection by laser-induced fluorescence. A further peculiarity concerns the development of automatized as well as in-capillary derivatization that can be performed using the commercially available instrumentation. The majority of the methods considered deal with the derivatization of amine group in small molecules (in particular, amino acids) as well as in proteins and peptides. Applications are also addressed to chiral analysis and for trapping unstable and reactive small molecules and inorganic ions. The analysis of proteins and saccharides in glycomics, have been covered in dedicated sections.

Recent applications of the derivatization techniques in capillary electrophoresis / R. Gotti, B. Pasquini, S. Orlandini, S. Furlanetto. - STAMPA. - 1:(2023), pp. 100003.1-100003.12. [10.1016/j.jpbao.2023.100003]

Recent applications of the derivatization techniques in capillary electrophoresis

B. Pasquini;S. Orlandini;S. Furlanetto
2023

Abstract

The main reasons for performing derivatization in capillary electrophoresis are largely the same as for liquid chromatography, however there are specific aspects in electrokinetic separations where derivatization plays specific roles. The review is focused on the articles published in the past 5 years with the aim to highlight this unicity. Derivatization is mainly applied to improve the inherent low sensitivity of capillary electrophoresis when optical detection is used and the introduction of originally developed derivatization approaches have been addressed mainly to the detection by laser-induced fluorescence. A further peculiarity concerns the development of automatized as well as in-capillary derivatization that can be performed using the commercially available instrumentation. The majority of the methods considered deal with the derivatization of amine group in small molecules (in particular, amino acids) as well as in proteins and peptides. Applications are also addressed to chiral analysis and for trapping unstable and reactive small molecules and inorganic ions. The analysis of proteins and saccharides in glycomics, have been covered in dedicated sections.
2023
1
1
12
R. Gotti, B. Pasquini, S. Orlandini, S. Furlanetto
File in questo prodotto:
File Dimensione Formato  
2023_Gotti-R._J. Pharm. Biomed. Anal. Open.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: DRM non definito
Dimensione 1.08 MB
Formato Adobe PDF
1.08 MB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1300582
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact