Neuro-rehabilitative research is developing novel strategies to enhance the effectiveness of rehabilitation after stroke by combining physical therapy and plasticizing treatment. Previous studies have shown that the peri-infarct area spontaneously undergoes active remodeling after stroke and this lead to the remapping of cortical areas. It was already reported that repeated neuronal optogenetic stimulation of the peri-lesioned area induces a significant improvement in cerebral blood flow and neurovascular coupling response enhances the recovery of somatosensory cortical circuit function and limb sensorimotor abilities. Here we apply an optogenetic approach, that allows the manipulation of targeted cell populations in the peri-lesioned region, to promote the activation of peri-infarct neurons. We repeatedly stimulated Channelrhodopsin2-transfected neurons with a 473 nm laser five days a week for a month. We further combined optogenetic stimulation and motor training on a robotic platform (M-Platform). The robotic device for rehabilitation of the injured forelimb was integrated within a wide-field microscope, allowing simultaneous mesoscale calcium imaging of cortical activation on Thy1-GCaMP6f mice and recording of forces applied by the injured forelimb during the training sessions. Through behavioral analysis, we show that both strategies trigger a consistent improvement of forelimb functionality. Nevertheless, the combined therapy induces a significant and anticipated recovery of motor function compared to the single treatments.

All-Optical Rehabilitation Promotes Motor Recovery in a Mouse Model of Stroke / Conti, Emilia; Allegra Mascaro, Anna L.; Resta, Francesco; Sacconi, Leonardo; Pasquini, Maria; micera, silvestro; Pavone, Francesco S.. - ELETTRONICO. - (2018), pp. 0-0. (Intervento presentato al convegno Optics and the Brain) [10.1364/BRAIN.2018.BTh2C.2].

All-Optical Rehabilitation Promotes Motor Recovery in a Mouse Model of Stroke

Conti, Emilia;Allegra Mascaro, Anna L.;Resta, Francesco;Sacconi, Leonardo;Pasquini, Maria;Pavone, Francesco S.
2018

Abstract

Neuro-rehabilitative research is developing novel strategies to enhance the effectiveness of rehabilitation after stroke by combining physical therapy and plasticizing treatment. Previous studies have shown that the peri-infarct area spontaneously undergoes active remodeling after stroke and this lead to the remapping of cortical areas. It was already reported that repeated neuronal optogenetic stimulation of the peri-lesioned area induces a significant improvement in cerebral blood flow and neurovascular coupling response enhances the recovery of somatosensory cortical circuit function and limb sensorimotor abilities. Here we apply an optogenetic approach, that allows the manipulation of targeted cell populations in the peri-lesioned region, to promote the activation of peri-infarct neurons. We repeatedly stimulated Channelrhodopsin2-transfected neurons with a 473 nm laser five days a week for a month. We further combined optogenetic stimulation and motor training on a robotic platform (M-Platform). The robotic device for rehabilitation of the injured forelimb was integrated within a wide-field microscope, allowing simultaneous mesoscale calcium imaging of cortical activation on Thy1-GCaMP6f mice and recording of forces applied by the injured forelimb during the training sessions. Through behavioral analysis, we show that both strategies trigger a consistent improvement of forelimb functionality. Nevertheless, the combined therapy induces a significant and anticipated recovery of motor function compared to the single treatments.
2018
Optics and the Brain Conference papaers
Optics and the Brain
Conti, Emilia; Allegra Mascaro, Anna L.; Resta, Francesco; Sacconi, Leonardo; Pasquini, Maria; micera, silvestro; Pavone, Francesco S.
File in questo prodotto:
File Dimensione Formato  
All-Optical Rehabilitation Promotes Motor Recovery in a Mouse Model of Stroke.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: DRM non definito
Dimensione 292.15 kB
Formato Adobe PDF
292.15 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1306660
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact